Molecular evidence of SB100X-mediated transposition in vivo
| Tissue/transposition site sequences . | Chromosome location:hit from . | Sequence frequency . | Located gene . | Gene symbol . | Cancer-related gene . | Proximal TSS . | Distance to TSS, bp . |
|---|---|---|---|---|---|---|---|
| BM-12 | |||||||
| CAACTGTATATTGTCTAGTCCCTTAAGCGGAGCCCT | short sequence | 2 | NA | NA | NA | NA | NA |
| Spleen-12 | |||||||
| CAACTGTATAATTTTAGGTTACCATCTTCCATGGGGGAA | chr12:69,015,183 | 1 | Intronic | CNOT2 | CNOT2, NOT2 | chr12:68923454 | 91729 |
| CAACTGTAGCTTATTCAGATTTTGTATATACATAGAAAAT | chr1:225568155 | 1 | Intronic | CDC42BPA | CDC42BPA, PK428, MRCKA | chr1:225571573 | 3359 |
| CAACTGTACATTCCGACAGCCTGGGGAAATGGATCTTTG | chr3:58,431,902 | 1 | Intergenic | NA | NA | NA | NA |
| BM-18 | |||||||
| CAACTGTATGTCAAAATGCCCCTGTAGGCAGAACCTACA | multi | 3 | NA | NA | NA | NA | NA |
| CAACTGTACCAGGTACCTTCTCTGTGCCAGCCTCTTCCC | chr6:144,514,825 | 1 | Intronic | STX11 | STX11, FHL4, HPLH4, HLH4 | chr6:144513369 | 1456 |
| CAACTGTATGTCACAATGATCCCTGTAGGCAAAGCCTAG | multi | 2 | NA | NA | NA | NA | NA |
| Spleen-18 | |||||||
| CAACTGTATGTGTGGGTGAACCAGGTAGGAAGGTATGTG | chr12:62,625,888 | 1 | Intronic | SRGAP1 | SRGAP1, KIAA1304 | chr12:62524327 | 101561 |
| CAACTGTATATAGTATCTGGAGTTTCCTAGTCCCTTAAGC | multi | 1 | NA | NA | NA | NA | NA |
| CAACTGTATATAGTATCTGAAGTTTCCTAGTCCCTTAAGC | chr4:154,192,090 | 3 | Intergenic | NA | NA | NA | NA |
| CAACTGTATCAATGATAATGAAGAAGCTACAACTACATAT | chr9:93,833,074 | 3 | Intergenic | NA | NA | NA | NA |
| CAACTGTATCAAATGTAAGATACTAGTCCCTTAAGCGGAG | chr17:59,141,036 | 1 | Intronic | LYK5; GH1 | LYK5, PMSE; GH1, GHN | not available | NA |
| BM-14 | |||||||
| CAACTGTACTTGACCCCATTAAAATGTCAGTAAGTTGAATT | chr3:103,263,186 | 2 | Intergenic | NA | NA | NA | NA |
| CAACTGTACCAGGTACCTTCTCTGTGCCAGCCTCTTCCCT | multi | 1 | NA | NA | NA | NA | NA |
| CAACTGTAGCTTATTCAGATTTTGTATATACATAGAAAATA | chr1:225568155 | 1 | Intronic | CDC42BPA | CDC42BPA, PK428, MRCKA | chr1:225571573 | 3359 |
| Tissue/transposition site sequences . | Chromosome location:hit from . | Sequence frequency . | Located gene . | Gene symbol . | Cancer-related gene . | Proximal TSS . | Distance to TSS, bp . |
|---|---|---|---|---|---|---|---|
| BM-12 | |||||||
| CAACTGTATATTGTCTAGTCCCTTAAGCGGAGCCCT | short sequence | 2 | NA | NA | NA | NA | NA |
| Spleen-12 | |||||||
| CAACTGTATAATTTTAGGTTACCATCTTCCATGGGGGAA | chr12:69,015,183 | 1 | Intronic | CNOT2 | CNOT2, NOT2 | chr12:68923454 | 91729 |
| CAACTGTAGCTTATTCAGATTTTGTATATACATAGAAAAT | chr1:225568155 | 1 | Intronic | CDC42BPA | CDC42BPA, PK428, MRCKA | chr1:225571573 | 3359 |
| CAACTGTACATTCCGACAGCCTGGGGAAATGGATCTTTG | chr3:58,431,902 | 1 | Intergenic | NA | NA | NA | NA |
| BM-18 | |||||||
| CAACTGTATGTCAAAATGCCCCTGTAGGCAGAACCTACA | multi | 3 | NA | NA | NA | NA | NA |
| CAACTGTACCAGGTACCTTCTCTGTGCCAGCCTCTTCCC | chr6:144,514,825 | 1 | Intronic | STX11 | STX11, FHL4, HPLH4, HLH4 | chr6:144513369 | 1456 |
| CAACTGTATGTCACAATGATCCCTGTAGGCAAAGCCTAG | multi | 2 | NA | NA | NA | NA | NA |
| Spleen-18 | |||||||
| CAACTGTATGTGTGGGTGAACCAGGTAGGAAGGTATGTG | chr12:62,625,888 | 1 | Intronic | SRGAP1 | SRGAP1, KIAA1304 | chr12:62524327 | 101561 |
| CAACTGTATATAGTATCTGGAGTTTCCTAGTCCCTTAAGC | multi | 1 | NA | NA | NA | NA | NA |
| CAACTGTATATAGTATCTGAAGTTTCCTAGTCCCTTAAGC | chr4:154,192,090 | 3 | Intergenic | NA | NA | NA | NA |
| CAACTGTATCAATGATAATGAAGAAGCTACAACTACATAT | chr9:93,833,074 | 3 | Intergenic | NA | NA | NA | NA |
| CAACTGTATCAAATGTAAGATACTAGTCCCTTAAGCGGAG | chr17:59,141,036 | 1 | Intronic | LYK5; GH1 | LYK5, PMSE; GH1, GHN | not available | NA |
| BM-14 | |||||||
| CAACTGTACTTGACCCCATTAAAATGTCAGTAAGTTGAATT | chr3:103,263,186 | 2 | Intergenic | NA | NA | NA | NA |
| CAACTGTACCAGGTACCTTCTCTGTGCCAGCCTCTTCCCT | multi | 1 | NA | NA | NA | NA | NA |
| CAACTGTAGCTTATTCAGATTTTGTATATACATAGAAAATA | chr1:225568155 | 1 | Intronic | CDC42BPA | CDC42BPA, PK428, MRCKA | chr1:225571573 | 3359 |
Genomic DNA was extracted from total mouse bone marrow cells and spleen cells at 12–14 weeks after transplantation with SB transposon + SB100X transfected CD34+ HPCs.
Bold letters indicate transposon sequence; BM-12, bone marrow cells from the mouse 12; multi, multi chromosme locations due to the sequence similarity; NA, not applicable; and TSS, transcriptional start site.