Table 2 Quantitation of mAb binding to... | American Society of Hematology

Table 2

Quantitation of mAb binding to intact erythrocytes and ghosts after incubation in acidified NHS

Treatment	Al647 mAb H17 binding to		Al488 mAb 1H8 binding to
Treatment	Erythrocytes	Ghosts	Erythrocytes	Ghosts
Acidified NHS	520	13 000	700	37 600
Acidified NHS + 5 μg/mL Al647 mAb H17	96	2100	770	23 200

Treatment	Al647 mAb H17 binding to		Al488 mAb 1H8 binding to
Treatment	Erythrocytes	Ghosts	Erythrocytes	Ghosts
Acidified NHS	520	13 000	700	37 600
Acidified NHS + 5 μg/mL Al647 mAb H17	96	2100	770	23 200

Binding expressed as molecules of equivalent soluble fluorochrome (MESF).

mAb indicates monoclonal antibody.