Table 1 Kinetic parameters of leukocyte... | American Society of Hematology

Table 1

Kinetic parameters of leukocyte proteases for cleavage of rFRETS-VWF73

Construct	k_cat, s⁻¹	k_m, ×10⁻⁶ M	k_cat/k_m, 10⁶ M⁻¹s⁻¹
ADAMTS13	4.43 ± 0.52	5.81 ± 1.61	0.76
Elastase	2.97 ± 0.43	1.16 ± 0.65	1.70
Cathepsin G	4.99 ± 0.39	0.76 ± 0.27	6.57
PR3	12.07 ± 1.18	2.56 ± 0.78	4.71
MMP9	2.02 ± 0.48	4.80 ± 0.82	0.42

Construct	k_cat, s⁻¹	k_m, ×10⁻⁶ M	k_cat/k_m, 10⁶ M⁻¹s⁻¹
ADAMTS13	4.43 ± 0.52	5.81 ± 1.61	0.76
Elastase	2.97 ± 0.43	1.16 ± 0.65	1.70
Cathepsin G	4.99 ± 0.39	0.76 ± 0.27	6.57
PR3	12.07 ± 1.18	2.56 ± 0.78	4.71
MMP9	2.02 ± 0.48	4.80 ± 0.82	0.42

The data represent the means and SD of at least 3 independent experiments.

k_cat indicates catalytic constant equal to V_max/[E]; and k_m, Michaelis-Menten constant.