2−DDCT data analysis
| Description . | Average CT, TF . | Average CT, GAPDH . | DCT . | DDCT . | 2−DDCT . | Expression difference* . |
|---|---|---|---|---|---|---|
| Glomeruli from untreated mice | 31.5 | 20.3 | 11.2 ± 1.0 | 0 ± 0.65 | — | — |
| Glomeruli from FB1-treated mice | 28.3 | 21.3 | 7.0 ± 1.5 | −4.2 ± 0.6 | 18.4 ± 1.0 | ×18.4 (2 > 1)† |
| Glomeruli from FC1-treated mice | 28.7 | 21.5 | 7.2 ± 1.8 | −4 ± 1.0 | 16.0 ± 0.8 | ×16.0 (3 > 1)† |
| Glomeruli from aPL-IgG-treated mice | 27.9 | 22.1 | 6.8 ± 1.8 | −4.4 ± 1.2 | 21.1 ± 1.3 | ×21.1 (4 > 1)† |
| Description . | Average CT, TF . | Average CT, GAPDH . | DCT . | DDCT . | 2−DDCT . | Expression difference* . |
|---|---|---|---|---|---|---|
| Glomeruli from untreated mice | 31.5 | 20.3 | 11.2 ± 1.0 | 0 ± 0.65 | — | — |
| Glomeruli from FB1-treated mice | 28.3 | 21.3 | 7.0 ± 1.5 | −4.2 ± 0.6 | 18.4 ± 1.0 | ×18.4 (2 > 1)† |
| Glomeruli from FC1-treated mice | 28.7 | 21.5 | 7.2 ± 1.8 | −4 ± 1.0 | 16.0 ± 0.8 | ×16.0 (3 > 1)† |
| Glomeruli from aPL-IgG-treated mice | 27.9 | 22.1 | 6.8 ± 1.8 | −4.4 ± 1.2 | 21.1 ± 1.3 | ×21.1 (4 > 1)† |
Relative quantification of TF between glomeruli from FB1- and FC1-treated mice and glomeruli from untreated mice. The comparison was performed in pairs using the same target gene and the same glomeruli samples.
CT indicates threshold value; DCT, average target CT − average GAPDH CT; DDCT (for the same target gene), average DCT glomeruli from FB1- or FC1-treated mice − average DCT glomeruli from untreated mice (DDCT = 0 when the glomeruli suspension of interest is used as calibrator); —, not applicable; and 2−DDCT, normalized target gene amount (TF) relative to target gene amount in glomeruli from untreated mice.
P < .001; n = 4 to 8 mice/group.
Different from glomeruli from untreated mice (P < .001).