Table 1 Primers used for generation of... | American Society of Hematology

Table 1

Primers used for generation of ADAMTS13 mutants

Primer name	Sequences
1. Del6aa	F: CAG GAA GAT GCT GAC ATC CAG GTT TAC- - -TAT GGC AAC CTC ACC CGC CCA GAC AT
1. Del6aa	R: GAT GTC TGG GCG GGT GAG GTT GCC ATA- - -GTA AAC CTG GAT GTC AGC ATC TTC CTG
2. R659A	F: GCT GAC ATC CAG GTT TAC GCG CGG TAT GGC GAG GAG TAT
2. R659A	R: ATA CTC CTC GCC ATA CCG CGC GTA AAC CTG GAT GTC AGC
3. R660A	F: GAC ATC CAG GTT TAC AGG GCG TAT GGC GAG GAG TAT GGC
3. R660A	R: GCC ATA CTC CTC GCC ATA CGC CCT GTA AAC CTG GAT GTC
4. Y661A	F: ATC CAG GTT TAC AGG CGG GCT GGC GAG GAG TAT GGC AAC
4. Y661A	R: GTT GCC ATA CTC CTC GCC AGC CCG CCT GTA AAC CTG GAT
5. G662A	F: CAG GTT TAC AGG CGG TAT GCC GAG GAG TAT GGC AAC CTC
5. G662A	R: GAG GTT GCC ATA CTC CTC GGC ATA CCG CCT GTA AAC CTG
6. E663A	F: TAC AGG CGG TAT GGC GCG GAG TAT GGC AAC CTC
6. E663A	R: GAG GTT GCC ATA CTC CGC GCC ATA CCG CCT GTA
7. E664A	F: TAC AGG CGG TAT GGC GAG GCG TAT GGC AAC CTC ACC CGC
7. E664A	R: GCG GGT GAG GTT GCC ATA CGC CTC GCC ATA CCG CCT GTA

Primer name	Sequences
1. Del6aa	F: CAG GAA GAT GCT GAC ATC CAG GTT TAC- - -TAT GGC AAC CTC ACC CGC CCA GAC AT
1. Del6aa	R: GAT GTC TGG GCG GGT GAG GTT GCC ATA- - -GTA AAC CTG GAT GTC AGC ATC TTC CTG
2. R659A	F: GCT GAC ATC CAG GTT TAC GCG CGG TAT GGC GAG GAG TAT
2. R659A	R: ATA CTC CTC GCC ATA CCG CGC GTA AAC CTG GAT GTC AGC
3. R660A	F: GAC ATC CAG GTT TAC AGG GCG TAT GGC GAG GAG TAT GGC
3. R660A	R: GCC ATA CTC CTC GCC ATA CGC CCT GTA AAC CTG GAT GTC
4. Y661A	F: ATC CAG GTT TAC AGG CGG GCT GGC GAG GAG TAT GGC AAC
4. Y661A	R: GTT GCC ATA CTC CTC GCC AGC CCG CCT GTA AAC CTG GAT
5. G662A	F: CAG GTT TAC AGG CGG TAT GCC GAG GAG TAT GGC AAC CTC
5. G662A	R: GAG GTT GCC ATA CTC CTC GGC ATA CCG CCT GTA AAC CTG
6. E663A	F: TAC AGG CGG TAT GGC GCG GAG TAT GGC AAC CTC
6. E663A	R: GAG GTT GCC ATA CTC CGC GCC ATA CCG CCT GTA
7. E664A	F: TAC AGG CGG TAT GGC GAG GCG TAT GGC AAC CTC ACC CGC
7. E664A	R: GCG GGT GAG GTT GCC ATA CGC CTC GCC ATA CCG CCT GTA

The dotted line between nucleotides indicates the portion being deleted; the nucleotides for a single amino acid substitution are underlined.