Results of transplantations from CD34−, CD34dim, and CD34+ fractions of AML into immunodeficient mice
| Sample . | Time, weeks . | CD34− . | CD34+ . | CD34dim . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose × 106 . | MNC dose × 106 . | Mice with AML/mice total . | Dose × 103 . | MNC dose × 106 . | Mice with AML/mice total . | Dose × 103 . | MNC dose × 106 . | Mice with AML/mice total . | ||
| 1 | 12-13 | 24 | 24 | 2/2* (2.8%) | 7.8 | 5.5 | 0/3† | NA | NA | NA |
| 1 | 12 | 5 | 5 | 0/2 | ||||||
| 2 | 12 | 21 | 21 | 2/2* (1.2%) | NT | NT | NT | NA | NA | NA |
| 7 | 9 | ‡ | ‡ | ‡ | 2.5 | 5.2 | 0/1 | 11 | 9.2 | 0/1 |
| 8 | 12 | 5.4 | 5.4 | 2/3* (0.1%) | 4.2 | 19 | 0/1† | 5 | 5.2 | 0/2 |
| 9 | 13-15 | 13 | 13 | 4/4* (68%) | 8.4 | 15 | 0/1 | 400 | 64 | 0/1 |
| WT1 | 9 | 6 | 6 | 1/1 (60%) | 3.2 | 6 | 0/1 | NA | NA | NA |
| Sample . | Time, weeks . | CD34− . | CD34+ . | CD34dim . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose × 106 . | MNC dose × 106 . | Mice with AML/mice total . | Dose × 103 . | MNC dose × 106 . | Mice with AML/mice total . | Dose × 103 . | MNC dose × 106 . | Mice with AML/mice total . | ||
| 1 | 12-13 | 24 | 24 | 2/2* (2.8%) | 7.8 | 5.5 | 0/3† | NA | NA | NA |
| 1 | 12 | 5 | 5 | 0/2 | ||||||
| 2 | 12 | 21 | 21 | 2/2* (1.2%) | NT | NT | NT | NA | NA | NA |
| 7 | 9 | ‡ | ‡ | ‡ | 2.5 | 5.2 | 0/1 | 11 | 9.2 | 0/1 |
| 8 | 12 | 5.4 | 5.4 | 2/3* (0.1%) | 4.2 | 19 | 0/1† | 5 | 5.2 | 0/2 |
| 9 | 13-15 | 13 | 13 | 4/4* (68%) | 8.4 | 15 | 0/1 | 400 | 64 | 0/1 |
| WT1 | 9 | 6 | 6 | 1/1 (60%) | 3.2 | 6 | 0/1 | NA | NA | NA |
NA indicates not applicable as these samples lack the CD34dim population; NT, not tested; and WT1, wild-type NPM AML sample 1. The figures in parentheses indicate the mean percentage of AML in the mouse bone marrow.
Sorted human cells from mouse bone marrow were shown to contain mutated NPM by quantitative PCR.
Normal multilineage engraftment observed; the DNA from the grafts contained wild-type NPM.
The LICs from sample 7 are found in the CD34− fraction (Table 4).