Table 1 Effect of substitutions of... | American Society of Hematology

Table 1

Effect of substitutions of αIIb-Trp110 and -Phe171 on inside-out and outside-in signaling–related functions of αIIbβ3

αIIb substitution	Surface expression	Soluble fibrinogen binding	Percent mature of total αIIb formation	Outside-in signaling–related functions*		Stability in EDTA
αIIb substitution	Surface expression	Soluble fibrinogen binding	Percent mature of total αIIb formation	Adhesion to immobilized fibrinogen	Clot retraction	Stability in EDTA
Trp110Ala	Reduced	Negligible	Reduced	ND	ND	ND
Phe171Ala	Reduced	Reduced†	Reduced	ND	ND	ND
Trp110Ala and Phe171Ala	Negligible	Negligible	Negligible	ND	ND	ND
Trp110Leu or Trp110Arg	Normal	Normal	Normal	Impaired	Impaired	Reduced
Phe171Ile or Phe171Val	Reduced	negligible	Reduced	ND	ND	ND

αIIb substitution	Surface expression	Soluble fibrinogen binding	Percent mature of total αIIb formation	Outside-in signaling–related functions*		Stability in EDTA
αIIb substitution	Surface expression	Soluble fibrinogen binding	Percent mature of total αIIb formation	Adhesion to immobilized fibrinogen	Clot retraction	Stability in EDTA
Trp110Ala	Reduced	Negligible	Reduced	ND	ND	ND
Phe171Ala	Reduced	Reduced†	Reduced	ND	ND	ND
Trp110Ala and Phe171Ala	Negligible	Negligible	Negligible	ND	ND	ND
Trp110Leu or Trp110Arg	Normal	Normal	Normal	Impaired	Impaired	Reduced
Phe171Ile or Phe171Val	Reduced	negligible	Reduced	ND	ND	ND

ND indicates not done.

Outside-in signaling–related functions were examined only when there was adequate surface expression.

Reduced to the same extent of αIIbβ3 surface expression.