Table 2 Characteristics of EBV-specific... | American Society of Hematology

Table 2

Characteristics of EBV-specific T-cell preparations used for therapy

Patient	Total viable cells administered	Administered cells/kg			CD8⁺, %*	CD8⁺ IFN-γ⁺, %*	CD4⁺, %*	CD4⁺ IFN-γ⁺, %*	CD14⁺, %*	CD14⁻CD8⁻CD4⁻, %*
Patient	Total viable cells administered	Total	CD8⁺	CD4⁺	CD8⁺, %*	CD8⁺ IFN-γ⁺, %*	CD4⁺, %*	CD4⁺ IFN-γ⁺, %*	CD14⁺, %*	CD14⁻CD8⁻CD4⁻, %*
1	1.3 × 10⁶	1.8 × 10⁴	1.5 × 10³	2.0 × 10³	8.2	3.1	10.9	1.9	41.4	39.5
2	3.9 × 10⁶	7.1 × 10⁴	15.5 × 10³	14.9 × 10³	21.8	12.4	21.0	1.5	13.0	44.2
3	5.2 × 10⁶	7.4 × 10⁴	3.0 × 10³	3.1 × 10³	4.0	3.2	4.2	2.9	74.9	16.8
4	0.3 × 10⁶	0.4 × 10⁴	0.6 × 10³	0.9 × 10³	14.7	7.5	21.2	5.1	25.5	38.6
5	6.1 × 10⁶	8.6 × 10⁴	0.7 × 10³	2.9 × 10³	0.8	0.3	3.4	0.5	34.7	61.1
6	7.4 × 10⁶	9.7 × 10⁴	0.5 × 10³	1.3 × 10³	0.5	0.2	1.3	0.3	69.8	28.4

Patient	Total viable cells administered	Administered cells/kg			CD8⁺, %*	CD8⁺ IFN-γ⁺, %*	CD4⁺, %*	CD4⁺ IFN-γ⁺, %*	CD14⁺, %*	CD14⁻CD8⁻CD4⁻, %*
Patient	Total viable cells administered	Total	CD8⁺	CD4⁺	CD8⁺, %*	CD8⁺ IFN-γ⁺, %*	CD4⁺, %*	CD4⁺ IFN-γ⁺, %*	CD14⁺, %*	CD14⁻CD8⁻CD4⁻, %*
1	1.3 × 10⁶	1.8 × 10⁴	1.5 × 10³	2.0 × 10³	8.2	3.1	10.9	1.9	41.4	39.5
2	3.9 × 10⁶	7.1 × 10⁴	15.5 × 10³	14.9 × 10³	21.8	12.4	21.0	1.5	13.0	44.2
3	5.2 × 10⁶	7.4 × 10⁴	3.0 × 10³	3.1 × 10³	4.0	3.2	4.2	2.9	74.9	16.8
4	0.3 × 10⁶	0.4 × 10⁴	0.6 × 10³	0.9 × 10³	14.7	7.5	21.2	5.1	25.5	38.6
5	6.1 × 10⁶	8.6 × 10⁴	0.7 × 10³	2.9 × 10³	0.8	0.3	3.4	0.5	34.7	61.1
6	7.4 × 10⁶	9.7 × 10⁴	0.5 × 10³	1.3 × 10³	0.5	0.2	1.3	0.3	69.8	28.4

Subpopulations were quantified immediately after IFN-γ capture–based cell isolation by staining with fluorescent monoclonal antibodies specific for CD4, CD8, CD14, and IFN-γ. Staining was evaluated by flow cytometry. Because of transient down-regulation of T-cell markers after activation and partial blockade of IFN-γ epitopes, this analysis may underrepresent the true proportions of T cells and IFN-γ⁺ cells (see text and Table 3).

Percentage of total viable cells.

View Large

This Feature Is Available To Subscribers Only