Reexpression of T-cell markers after IFN-γ–based isolation of EBV-specific T cells
| Donor . | CD8+, %* . | Increase in CD8+ cells 40 vs 0 h . | CD4+, %* . | Increase in CD4+ cells 40 vs 0 h . | ||||
|---|---|---|---|---|---|---|---|---|
| 0 h . | 20 h . | 40 h . | 0 h . | 20 h . | 40 h . | |||
| A | 5.4 | 11.8 | 11.7 | 2.2 | 3.6 | 9.5 | 8.4 | 2.3 |
| B | 6.4 | 13.1 | 16.3 | 2.5 | 8.4 | 30.5 | 41.7 | 5.0 |
| C | 16.0 | ND | 36 | 2.3 | 6.9 | ND | 17.4 | 2.5 |
| D | 7.1 | ND | 11 | 1.5 | 6.5 | ND | 17 | 2.6 |
| Mean ± SD | 2.1 ± 0.4 | 3.1 ± 1.2 | ||||||
| Donor . | CD8+, %* . | Increase in CD8+ cells 40 vs 0 h . | CD4+, %* . | Increase in CD4+ cells 40 vs 0 h . | ||||
|---|---|---|---|---|---|---|---|---|
| 0 h . | 20 h . | 40 h . | 0 h . | 20 h . | 40 h . | |||
| A | 5.4 | 11.8 | 11.7 | 2.2 | 3.6 | 9.5 | 8.4 | 2.3 |
| B | 6.4 | 13.1 | 16.3 | 2.5 | 8.4 | 30.5 | 41.7 | 5.0 |
| C | 16.0 | ND | 36 | 2.3 | 6.9 | ND | 17.4 | 2.5 |
| D | 7.1 | ND | 11 | 1.5 | 6.5 | ND | 17 | 2.6 |
| Mean ± SD | 2.1 ± 0.4 | 3.1 ± 1.2 | ||||||
Subpopulations were stained for FACS analysis immediately after IFN-γ capture–based cell isolation (0 hours) or after 20 hours or 40 hours of cultivation.
ND indicates not done.
Percentage of total viable cells.