Clinical and molecular characterization of patients with CLL
| CLL no. . | Progression status . | Binet stage . | Lymphocyte count, ×103/μL . | CD38* . | LPL . | Diagnostic and blood collection . | TFT, mo . | A/D† . | AID (Q-PCR) . | Percentage of CSR‡ . | VDJ clonal identity . | Mutational status§ . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1¶ | Progressor | C | 128.0 | + | + | 2000 | 9 | D | +/+ | 8.2 | Yes | UM |
| 2‖ | Progressor | B | 80.0 | + | + | 1999 | 18 | D | + | 4.1 | Yes | UM |
| 3¶ | Progressor | C | 31.00 | + | + | 1998 | 6 | D | +/+ | 5.4 | Yes | UM |
| 4¶ | Progressor | B | 105.00 | + | + | 2000 | 8 | D | +/+ | 6.4 | Yes | UM |
| 5** | Indolent | A | 20.00 | + | + | 1999 | N/T | A | − | 1.3 | N/A | UM |
| 6** | Progressor | B | 32.50 | − | + | 2001 | N/T | A | − | 0.3 | N/A | UM |
| 7‖ | Indolent | B | 17.00 | + | − | 2000 | N/T | A | + | 2.7 | Yes | UM |
| 8‖ | Progressor | A | 70.00 | − | − | 2002 | 42 | A | + | 1.6 | N/A | UM |
| 9‖ | Progressor | B | 60.00 | + | + | 2005 | 12 | A | + | 4 | Yes | UM |
| 10‖ | Indolent | A | 10.00 | + | + | 1999 | N/T | A | + | 0.7 | N/A | UM |
| 11‖ | Progressor | C | 88.00 | + | + | 2001 | 15 | A | + | 3.8 | Yes | UM |
| 12‖ | Progressor | B | 54.00 | + | + | 2001 | 6 | A | + | 4.5 | Yes | UM |
| 13‖ | Indolent | A | 20.00 | − | + | 2005 | 54 | A | + | 1.3 | No | UM |
| 14‖ | Indolent | A | 7.00 | + | + | 2006 | 36 | A | + | 3.3 | Yes | UM |
| 15** | Indolent | A | 20.40 | + | + | 2004 | N/T | A | − | 0.3 | No | UM |
| 16‖ | Progressor | B | 148.00 | + | + | 2005 | 3 | A | + | 1.4 | No | UM |
| 17‖ | Progressor | A | 54.50 | − | + | 2007 | 17 | A | + | 2.5 | No | UM |
| 18†† | Indolent | A | 15.00 | + | + | 2006 | 12 | A | +/+ | 5.9 | Yes | UM |
| 19** | Indolent | A | 11.00 | + | + | 2005 | N/T | A | − | 0.6 | No | UM |
| 20†† | Progressor | C | 220.00 | + | + | 2007 | 3 | D | +/+ | 7.4 | Yes | UM |
| 21** | Indolent | A | 8.90 | + | − | 2008 | N/T | A | − | 1.1 | No | UM |
| 22‖ | Progressor | B | 17.80 | + | + | 2008 | 11 | A | + | 3.5 | Yes | UM |
| 23‖ | Indolent | A | 28.00 | − | + | 2008 | N/T | A | + | 4.1 | No | UM |
| 24†† | Progressor | A | 80.00 | + | + | 2009 | 10 | A | +/+ | 5.5 | Yes | UM |
| 25‖ | Progressor | A | 10.50 | + | + | 2005 | N/T | A | + | 1 | N/A | UM |
| 26** | Progressor | A | 17.20 | − | − | 1999 | N/T | A | − | 0.9 | N/A | Mut |
| 27** | Progressor | A | 14.00 | − | − | 2000 | N/T | A | − | 0.4 | N/A | Mut |
| 28** | Progressor | A | 5.00 | + | − | 1989 | N/T | A | − | 0.6 | No | Mut |
| 29** | Progressor | A | 11.60 | + | − | 2001 | N/T | A | − | 0.1 | N/A | Mut |
| 30‖ | Progressor | A | 18.00 | − | − | 1999 | N/T | A | + | 1.8 | Yes | Mut |
| 31** | Progressor | B | 38.00 | + | − | 2000 | N/T | D | − | 0.5 | N/A | Mut |
| 32** | Indolent | A | 47.60 | − | − | 2002 | N/T | A | − | 2.5 | No | Mut |
| 33** | Indolent | A | 15.40 | − | − | 2006 | N/T | A | − | 0.8 | No | Mut |
| 34‖ | Indolent | A | 15.00 | − | + | 2007 | N/T | A | + | 0.5 | N/A | Mut |
| 35** | Indolent | A | 10.20 | − | + | 2006 | 25 | A | − | 1.2 | No | Mut |
| 36** | Indolent | A | 27.60 | − | + | 2007 | N/T | A | − | 0.4 | N/A | Mut |
| 37** | Indolent | A | 15.00 | − | − | 2006 | N/T | A | − | 0.3 | No | Mut |
| 38‖ | Indolent | A | 12.80 | − | + | 2004 | N/T | A | + | 2.1 | No | Mut |
| 39** | Indolent | A | 15.60 | − | + | 2006 | N/T | A | − | 0.5 | No | Mut |
| 40** | Indolent | A | 12.00 | − | + | 1997 | N/T | A | − | 0.1 | N/A | Mut |
| 41** | Progressor | C | 18.00 | − | + | 2007 | 6 | D | − | 1.0 | N/A | Mut |
| 42‖ | Indolent | A | 3.40 | − | − | 2008 | N/T | A | + | 1.7 | Yes | Mut |
| 43** | Indolent | A | 28.40 | − | + | 2007 | N/T | A | − | 2.0 | No | Mut |
| 44‖ | Indolent | A | 8.00 | − | − | 2006 | N/T | A | + | 2.2 | No | Mut |
| 45** | Progressor | B | 105.00 | − | + | 2007 | 12 | A | − | 2.0 | No | Mut |
| 46** | Indolent | A | 2.47 | − | − | 2008 | N/T | A | − | 0.1 | N/A | Mut |
| 47‖ | Indolent | C | 5.00 | − | − | 2009 | N/T | A | + | 1.8 | No | Mut |
| 48** | Indolent | A | 12.60 | − | − | 2008 | N/T | A | − | 2.1 | No | Mut |
| 49** | Indolent | A | 5.80 | − | − | 2008 | N/T | A | − | 1.1 | N/A | Mut |
| 50‖ | Indolent | A | 11.50 | − | − | 2008 | N/T | A | + | 2.1 | Yes | Mut |
| CLL no. . | Progression status . | Binet stage . | Lymphocyte count, ×103/μL . | CD38* . | LPL . | Diagnostic and blood collection . | TFT, mo . | A/D† . | AID (Q-PCR) . | Percentage of CSR‡ . | VDJ clonal identity . | Mutational status§ . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1¶ | Progressor | C | 128.0 | + | + | 2000 | 9 | D | +/+ | 8.2 | Yes | UM |
| 2‖ | Progressor | B | 80.0 | + | + | 1999 | 18 | D | + | 4.1 | Yes | UM |
| 3¶ | Progressor | C | 31.00 | + | + | 1998 | 6 | D | +/+ | 5.4 | Yes | UM |
| 4¶ | Progressor | B | 105.00 | + | + | 2000 | 8 | D | +/+ | 6.4 | Yes | UM |
| 5** | Indolent | A | 20.00 | + | + | 1999 | N/T | A | − | 1.3 | N/A | UM |
| 6** | Progressor | B | 32.50 | − | + | 2001 | N/T | A | − | 0.3 | N/A | UM |
| 7‖ | Indolent | B | 17.00 | + | − | 2000 | N/T | A | + | 2.7 | Yes | UM |
| 8‖ | Progressor | A | 70.00 | − | − | 2002 | 42 | A | + | 1.6 | N/A | UM |
| 9‖ | Progressor | B | 60.00 | + | + | 2005 | 12 | A | + | 4 | Yes | UM |
| 10‖ | Indolent | A | 10.00 | + | + | 1999 | N/T | A | + | 0.7 | N/A | UM |
| 11‖ | Progressor | C | 88.00 | + | + | 2001 | 15 | A | + | 3.8 | Yes | UM |
| 12‖ | Progressor | B | 54.00 | + | + | 2001 | 6 | A | + | 4.5 | Yes | UM |
| 13‖ | Indolent | A | 20.00 | − | + | 2005 | 54 | A | + | 1.3 | No | UM |
| 14‖ | Indolent | A | 7.00 | + | + | 2006 | 36 | A | + | 3.3 | Yes | UM |
| 15** | Indolent | A | 20.40 | + | + | 2004 | N/T | A | − | 0.3 | No | UM |
| 16‖ | Progressor | B | 148.00 | + | + | 2005 | 3 | A | + | 1.4 | No | UM |
| 17‖ | Progressor | A | 54.50 | − | + | 2007 | 17 | A | + | 2.5 | No | UM |
| 18†† | Indolent | A | 15.00 | + | + | 2006 | 12 | A | +/+ | 5.9 | Yes | UM |
| 19** | Indolent | A | 11.00 | + | + | 2005 | N/T | A | − | 0.6 | No | UM |
| 20†† | Progressor | C | 220.00 | + | + | 2007 | 3 | D | +/+ | 7.4 | Yes | UM |
| 21** | Indolent | A | 8.90 | + | − | 2008 | N/T | A | − | 1.1 | No | UM |
| 22‖ | Progressor | B | 17.80 | + | + | 2008 | 11 | A | + | 3.5 | Yes | UM |
| 23‖ | Indolent | A | 28.00 | − | + | 2008 | N/T | A | + | 4.1 | No | UM |
| 24†† | Progressor | A | 80.00 | + | + | 2009 | 10 | A | +/+ | 5.5 | Yes | UM |
| 25‖ | Progressor | A | 10.50 | + | + | 2005 | N/T | A | + | 1 | N/A | UM |
| 26** | Progressor | A | 17.20 | − | − | 1999 | N/T | A | − | 0.9 | N/A | Mut |
| 27** | Progressor | A | 14.00 | − | − | 2000 | N/T | A | − | 0.4 | N/A | Mut |
| 28** | Progressor | A | 5.00 | + | − | 1989 | N/T | A | − | 0.6 | No | Mut |
| 29** | Progressor | A | 11.60 | + | − | 2001 | N/T | A | − | 0.1 | N/A | Mut |
| 30‖ | Progressor | A | 18.00 | − | − | 1999 | N/T | A | + | 1.8 | Yes | Mut |
| 31** | Progressor | B | 38.00 | + | − | 2000 | N/T | D | − | 0.5 | N/A | Mut |
| 32** | Indolent | A | 47.60 | − | − | 2002 | N/T | A | − | 2.5 | No | Mut |
| 33** | Indolent | A | 15.40 | − | − | 2006 | N/T | A | − | 0.8 | No | Mut |
| 34‖ | Indolent | A | 15.00 | − | + | 2007 | N/T | A | + | 0.5 | N/A | Mut |
| 35** | Indolent | A | 10.20 | − | + | 2006 | 25 | A | − | 1.2 | No | Mut |
| 36** | Indolent | A | 27.60 | − | + | 2007 | N/T | A | − | 0.4 | N/A | Mut |
| 37** | Indolent | A | 15.00 | − | − | 2006 | N/T | A | − | 0.3 | No | Mut |
| 38‖ | Indolent | A | 12.80 | − | + | 2004 | N/T | A | + | 2.1 | No | Mut |
| 39** | Indolent | A | 15.60 | − | + | 2006 | N/T | A | − | 0.5 | No | Mut |
| 40** | Indolent | A | 12.00 | − | + | 1997 | N/T | A | − | 0.1 | N/A | Mut |
| 41** | Progressor | C | 18.00 | − | + | 2007 | 6 | D | − | 1.0 | N/A | Mut |
| 42‖ | Indolent | A | 3.40 | − | − | 2008 | N/T | A | + | 1.7 | Yes | Mut |
| 43** | Indolent | A | 28.40 | − | + | 2007 | N/T | A | − | 2.0 | No | Mut |
| 44‖ | Indolent | A | 8.00 | − | − | 2006 | N/T | A | + | 2.2 | No | Mut |
| 45** | Progressor | B | 105.00 | − | + | 2007 | 12 | A | − | 2.0 | No | Mut |
| 46** | Indolent | A | 2.47 | − | − | 2008 | N/T | A | − | 0.1 | N/A | Mut |
| 47‖ | Indolent | C | 5.00 | − | − | 2009 | N/T | A | + | 1.8 | No | Mut |
| 48** | Indolent | A | 12.60 | − | − | 2008 | N/T | A | − | 2.1 | No | Mut |
| 49** | Indolent | A | 5.80 | − | − | 2008 | N/T | A | − | 1.1 | N/A | Mut |
| 50‖ | Indolent | A | 11.50 | − | − | 2008 | N/T | A | + | 2.1 | Yes | Mut |
LPL indicates lipoprotein lipase; TFT, time from initial diagnosis to first treatment for clinical progression; A/D, alive/dead; CSR, class switch recombination; UM, unmutated; Mut, mutated; N/T, no treatment; and N/A, no amplification.
Obtained results in cytometric assays, using a CD38 cutoff of 30%.
Related to decease.
Percentage of IgG+ and IgM+/IgG+ CLL B cells by cytometric studies.
Less than or equal to 2% difference from germline gene defined patients with UM disease, ≥ 2% difference defines patients with MUT.
High AID expression and CSR ≥ 5%.
Low AID expression and CSR ≤ 5%.
No AID expression and CSR ≤ 2.5%.