Ad5-induced gene expression in a liver of C57BL/6_WT mice (fold over Mock, 6 hours after injection)
| . | Mock . | Ad5-GFP . | Ad5-IX-dGFP . | Ad5-GFP-IX-dDAF4 . | Ad5-GFP-IX-dDAF4_REO . |
|---|---|---|---|---|---|
| ADAR* | 1.0 ± 0.3 | 2.2 ± 0.4† | 2.6 ± 0.2† | 1.9 ± 0.5† | 2.4 ± 0.8† |
| CD14* | 1.0 ± 0.2 | 6.0 ± 1.5† | 10.0 ± 5.4‡ | 6.2 ± 3.2† | 3.4 ± 2.4 |
| CXCL-9 | 1.0 ± 0.2 | 10.7 ± 3.0† | 17.4 ± 5.7‡ | 10.6 ± 1.2† | 5.1 ± 1.3†§ |
| DAF | 1.0 ± 0.1 | 1.5 ± 0.5 | 1.2 ± 0.2 | 1.5 ± 0.3 | 1.1 ± 0.2 |
| e-Selectin | 1.0 ± 0.4 | 4.0 ± 0.6† | 5.7 ± 1.0‡ | 3.7 ± 1.3† | 3.5 ± 0.8† |
| ICAM* | 1.0 ± 0.3 | 4.2 ± 0.4† | 3.8 ± 0.8† | 3.6 ± 0.7† | 2.2 ± 0.3†§ |
| IFNα | 1.0 ± 0.4 | 1.3 ± 0.4 | 1.4 ± 0.1 | 0.8 ± 0.5 | 1.5 ± 0.5 |
| IFNβ | 1.0 ± 0.4 | 1.2 ± 0.4 | 1.5 ± 0.3 | 0.8 ± 0.5 | 1.7 ± 0.7 |
| IRF-7 | 1.0 ± 0.4 | 16.4 ± 3.6† | 19.6 ± 2.7† | 16.5 ± 5.0† | 10.9 ± 2.2†§ |
| IRF-8 | 1.0 ± 0.2 | 3.7 ± 1.1† | 3.6 ± 0.8† | 3.2 ± 1.1† | 1.9 ± 0.4§ |
| Jak-1 | 1.0 ± 0.3 | 2.3 ± 0.6† | 1.6 ± 0.3 | 1.8 ± 0.6 | 1.3 ± 0.3§ |
| Jak-3 | 1.0 ± 0.2 | 2.8 ± 0.8† | 1.7 ± 0.2 | 1.9 ± 1.0 | 1.2 ± 0.4§ |
| MyD88* | 1.0 ± 0.3 | 7.0 ± 1.4† | 8.9 ± 1.3† | 6.9 ± 1.9† | 4.0 ± 0.9†§ |
| NFκB-RelA | 1.0 ± 0.2 | 2.1 ± 0.4† | 2.6 ± 0.6† | 1.7 ± 0.4† | 1.2 ± 0.2§ |
| NOD-1 | 1.0 ± 0.2 | 2.4 ± 0.4† | 2.6 ± 0.3† | 2.7 ± 0.5† | 1.9 ± 0.3† |
| NOD-2 | 1.0 ± 0.4 | 2.0 ± 0.4† | 2.3 ± 0.2† | 1.5 ± 0.4 | 2.4 ± 0.7† |
| OAS-1a* | 1.0 ± 0.5 | 12.1 ± 1.0† | 14.5 ± 1.4† | 12.9 ± 4.8† | 5.9 ± 1.6†§ |
| SOCS-1* | 1.1 ± 0.7 | 12.6 ± 4.0† | 12.7 ± 4.1† | 11.7 ± 4.7† | 6.7 ± 2.3† |
| SOCS-3* | 1.1 ± 0.6 | 5.4 ± 1.8† | 5.8 ± 1.4† | 4.6 ± 0.9† | 4.0 ± 0.9† |
| TBK-1 | 1.0 ± 0.2 | 6.5 ± 0.8† | 6.1 ± 1.7† | 6.7 ± 1.9† | 3.5 ± 0.7†§ |
| TLR-2* | 1.0 ± 0.4 | 45.2 ± 14.3† | 86.7 ± 30.0‡ | 48.4 ± 16.0† | 29.3 ± 7.6† |
| TLR-3* | 1.0 ± 0.2 | 19.2 ± 2.9† | 29.7 ± 4.0‡ | 25.3 ± 7.0† | 15.0 ± 6.0† |
| TLR-6* | 1.0 ± 0.3 | 6.0 ± 1.6† | 4.2 ± 0.6† | 6.0 ± 2.6† | 3.0 ± 0.9§ |
| TLR-9* | 1.0 ± 0.2 | 2.0 ± 0.4† | 2.5 ± 0.3† | 1.9 ± 0.4† | 2.5 ± 0.8† |
| TRAF2bp* | 1.1 ± 0.5 | 16.3 ± 7.8† | 36.2 ± 6.3‡ | 19.7 ± 6.3† | 8.2 ± 1.9†§ |
| VCAM | 1.0 ± 0.4 | 2.1 ± 0.8 | 1.9 ± 0.1 | 1.5 ± 0.7 | 1.7 ± 0.6 |
| . | Mock . | Ad5-GFP . | Ad5-IX-dGFP . | Ad5-GFP-IX-dDAF4 . | Ad5-GFP-IX-dDAF4_REO . |
|---|---|---|---|---|---|
| ADAR* | 1.0 ± 0.3 | 2.2 ± 0.4† | 2.6 ± 0.2† | 1.9 ± 0.5† | 2.4 ± 0.8† |
| CD14* | 1.0 ± 0.2 | 6.0 ± 1.5† | 10.0 ± 5.4‡ | 6.2 ± 3.2† | 3.4 ± 2.4 |
| CXCL-9 | 1.0 ± 0.2 | 10.7 ± 3.0† | 17.4 ± 5.7‡ | 10.6 ± 1.2† | 5.1 ± 1.3†§ |
| DAF | 1.0 ± 0.1 | 1.5 ± 0.5 | 1.2 ± 0.2 | 1.5 ± 0.3 | 1.1 ± 0.2 |
| e-Selectin | 1.0 ± 0.4 | 4.0 ± 0.6† | 5.7 ± 1.0‡ | 3.7 ± 1.3† | 3.5 ± 0.8† |
| ICAM* | 1.0 ± 0.3 | 4.2 ± 0.4† | 3.8 ± 0.8† | 3.6 ± 0.7† | 2.2 ± 0.3†§ |
| IFNα | 1.0 ± 0.4 | 1.3 ± 0.4 | 1.4 ± 0.1 | 0.8 ± 0.5 | 1.5 ± 0.5 |
| IFNβ | 1.0 ± 0.4 | 1.2 ± 0.4 | 1.5 ± 0.3 | 0.8 ± 0.5 | 1.7 ± 0.7 |
| IRF-7 | 1.0 ± 0.4 | 16.4 ± 3.6† | 19.6 ± 2.7† | 16.5 ± 5.0† | 10.9 ± 2.2†§ |
| IRF-8 | 1.0 ± 0.2 | 3.7 ± 1.1† | 3.6 ± 0.8† | 3.2 ± 1.1† | 1.9 ± 0.4§ |
| Jak-1 | 1.0 ± 0.3 | 2.3 ± 0.6† | 1.6 ± 0.3 | 1.8 ± 0.6 | 1.3 ± 0.3§ |
| Jak-3 | 1.0 ± 0.2 | 2.8 ± 0.8† | 1.7 ± 0.2 | 1.9 ± 1.0 | 1.2 ± 0.4§ |
| MyD88* | 1.0 ± 0.3 | 7.0 ± 1.4† | 8.9 ± 1.3† | 6.9 ± 1.9† | 4.0 ± 0.9†§ |
| NFκB-RelA | 1.0 ± 0.2 | 2.1 ± 0.4† | 2.6 ± 0.6† | 1.7 ± 0.4† | 1.2 ± 0.2§ |
| NOD-1 | 1.0 ± 0.2 | 2.4 ± 0.4† | 2.6 ± 0.3† | 2.7 ± 0.5† | 1.9 ± 0.3† |
| NOD-2 | 1.0 ± 0.4 | 2.0 ± 0.4† | 2.3 ± 0.2† | 1.5 ± 0.4 | 2.4 ± 0.7† |
| OAS-1a* | 1.0 ± 0.5 | 12.1 ± 1.0† | 14.5 ± 1.4† | 12.9 ± 4.8† | 5.9 ± 1.6†§ |
| SOCS-1* | 1.1 ± 0.7 | 12.6 ± 4.0† | 12.7 ± 4.1† | 11.7 ± 4.7† | 6.7 ± 2.3† |
| SOCS-3* | 1.1 ± 0.6 | 5.4 ± 1.8† | 5.8 ± 1.4† | 4.6 ± 0.9† | 4.0 ± 0.9† |
| TBK-1 | 1.0 ± 0.2 | 6.5 ± 0.8† | 6.1 ± 1.7† | 6.7 ± 1.9† | 3.5 ± 0.7†§ |
| TLR-2* | 1.0 ± 0.4 | 45.2 ± 14.3† | 86.7 ± 30.0‡ | 48.4 ± 16.0† | 29.3 ± 7.6† |
| TLR-3* | 1.0 ± 0.2 | 19.2 ± 2.9† | 29.7 ± 4.0‡ | 25.3 ± 7.0† | 15.0 ± 6.0† |
| TLR-6* | 1.0 ± 0.3 | 6.0 ± 1.6† | 4.2 ± 0.6† | 6.0 ± 2.6† | 3.0 ± 0.9§ |
| TLR-9* | 1.0 ± 0.2 | 2.0 ± 0.4† | 2.5 ± 0.3† | 1.9 ± 0.4† | 2.5 ± 0.8† |
| TRAF2bp* | 1.1 ± 0.5 | 16.3 ± 7.8† | 36.2 ± 6.3‡ | 19.7 ± 6.3† | 8.2 ± 1.9†§ |
| VCAM | 1.0 ± 0.4 | 2.1 ± 0.8 | 1.9 ± 0.1 | 1.5 ± 0.7 | 1.7 ± 0.6 |
Medium dose of virus used for injection was 0.75 × 1011 vp/mouse. The numbers represent mean ± SD. Statistical analysis was completed using one-way analysis of variance with a Student-Newman-Keuls post hoc test; P < .05 was deemed a statistically significant difference. n = 4 for mock-injected groups, n = 5 for virus-injected groups.
Genes shown to be induced in C3-dependent manner after systemic Ad injection [2].
Significant differences compared to WT_Mock.
Significant induction of transcriptional activation compared to WT_Ad5-GFP group.
Significant reductions of transcriptional activation compared to WT_Ad5-GFP group.