Table 1 Phenotype of DC act and DC act... | American Society of Hematology

Table 1

Phenotype of DC_act and DC_act-Fb on coculture with fibroblasts

	DC_act	DC_act-Fb
HLA-DR, percentage	97.8 ± 1.3	96.8 ± 3
HLA-DR, mean	102 ± 39	92 ± 28
CD1c, percentage	48.6 ± 16	46.6 ± 15
iNOS, percentage	36 ± 3	27 ± 6.9
TNF-α, pg/mL	3020 ± 1608	1188 ± 502*
IL-23, pg/mL	210 ± 85	722 ± 160†
IL-12, pg/mL	38 ± 15	67 ± 26†
IL-6, pg/mL	24 250 ± 6339	120 092 ± 38 586†
IL-1β, pg/mL	178 ± 113	107 ± 74
TGF-β, pg/mL	168 ± 184	247 ± 350

	DC_act	DC_act-Fb
HLA-DR, percentage	97.8 ± 1.3	96.8 ± 3
HLA-DR, mean	102 ± 39	92 ± 28
CD1c, percentage	48.6 ± 16	46.6 ± 15
iNOS, percentage	36 ± 3	27 ± 6.9
TNF-α, pg/mL	3020 ± 1608	1188 ± 502*
IL-23, pg/mL	210 ± 85	722 ± 160†
IL-12, pg/mL	38 ± 15	67 ± 26†
IL-6, pg/mL	24 250 ± 6339	120 092 ± 38 586†
IL-1β, pg/mL	178 ± 113	107 ± 74
TGF-β, pg/mL	168 ± 184	247 ± 350

DCs were preactivated with LPS and afterward cocultured without (DC_act) and with fibroblasts (DC_act-Fb) overnight. HLA-DR (percentage positive cells and mean), CD11c, and iNOS (percentage positive cells) were measured by flow cytometry. IL-23, IL-12, IL-6, TGF-β, IL-1β, and TNF-α protein levels were measured by ELISA.

P < .05 vs DC_act (n = 3 independent experiments).

†

P < .001 vs DC_act (n = 3 independent experiments).

View Large

This Feature Is Available To Subscribers Only