Significantly enriched gene ontology terms for genes up-regulated in IFN-α and IL-4 DCs
| Treatment/GO system and GO category/terms . | Benjamini adjustment factor* . |
|---|---|
| IFN-α biologic processes | |
| Immune response | 2.4e-019 |
| Defense response | 4e-019 |
| Response to external stimuli | 4.7e-016 |
| Organismal physiologic process | 5.3e-014 |
| Response to pathogen/parasite | 1.6e-007 |
| Inflammatory response | 5.6e-006 |
| Innate immune response | 7.2e-006 |
| Response to stress | 1.7e-004 |
| Chemotaxis | 2.5e-003 |
| Physiologic process | 3e-002 |
| Apoptosis | 4.7e-002 |
| Antigen presentation | 1.3e-001 |
| Antigen processing | 1.4e-001 |
| IFN-α molecular functions | |
| Signal transducer activity | 3e-002 |
| Cytokine activity | 3e-002 |
| Chemokine activity | 4.7e-002 |
| Transmembrane receptor activity | 1e-001 |
| Oxidoreductase activity | 7.9e-003 |
| RNA binding | 1e + 000 |
| Catalytic activity | 1e + 000 |
| IL-4 molecular function | |
| Oxidoreductase activity | 7.9e-003 |
| RNA binding | 1e + 000 |
| Catalytic activity | 1e + 000 |
| IL-4 biologic processes | |
| Lipid metabolism | 3.9e-002 |
| Fatty acid metabolism | 1.6e-001 |
| Biosynthesis | 1e + 000 |
| Macromolecule biosynthesis | 1e + 000 |
| Carboxylic acid metabolism | 1e + 000 |
| Organic acid metabolism | 1e + 000 |
| IL-4 cellular components | |
| Membrane | 1e + 000 |
| Treatment/GO system and GO category/terms . | Benjamini adjustment factor* . |
|---|---|
| IFN-α biologic processes | |
| Immune response | 2.4e-019 |
| Defense response | 4e-019 |
| Response to external stimuli | 4.7e-016 |
| Organismal physiologic process | 5.3e-014 |
| Response to pathogen/parasite | 1.6e-007 |
| Inflammatory response | 5.6e-006 |
| Innate immune response | 7.2e-006 |
| Response to stress | 1.7e-004 |
| Chemotaxis | 2.5e-003 |
| Physiologic process | 3e-002 |
| Apoptosis | 4.7e-002 |
| Antigen presentation | 1.3e-001 |
| Antigen processing | 1.4e-001 |
| IFN-α molecular functions | |
| Signal transducer activity | 3e-002 |
| Cytokine activity | 3e-002 |
| Chemokine activity | 4.7e-002 |
| Transmembrane receptor activity | 1e-001 |
| Oxidoreductase activity | 7.9e-003 |
| RNA binding | 1e + 000 |
| Catalytic activity | 1e + 000 |
| IL-4 molecular function | |
| Oxidoreductase activity | 7.9e-003 |
| RNA binding | 1e + 000 |
| Catalytic activity | 1e + 000 |
| IL-4 biologic processes | |
| Lipid metabolism | 3.9e-002 |
| Fatty acid metabolism | 1.6e-001 |
| Biosynthesis | 1e + 000 |
| Macromolecule biosynthesis | 1e + 000 |
| Carboxylic acid metabolism | 1e + 000 |
| Organic acid metabolism | 1e + 000 |
| IL-4 cellular components | |
| Membrane | 1e + 000 |
IFN-α treatment induces overexpression of gene families linked to immunologic pathways, compared with IL-4 treatment enhancing metabolic pathways. IFN-α–induced gene categories show very high statistical significance in comparison with those modulated by IL-4 treatment.
DC indicates dendritic cell; GO, gene ontology; IFN-α, interferon-α; and IL-4, interleukin-4.
Benjamini adjustment factor was obtained by Benjamini-Hochberg correction of the statistical significance of effects estimated with multiple comparisons by controlling false discovery rate (FDR).