Table 1

Maturation status of DCs derived from patients with malignant melanoma

Patient no.SexAge, yStage*Distant metastasis*DC generationDEC-205, %MFI DEC-205§CD83, %MFI CD83§
Female 73 IV M1a Adherence 97 210 96 91 
Male 52 IV M1c Elutriation 98 244 97 50 
Female 65 IIIc — Adherence 96 238 93 59 
Male 66 IV M1b Adherence 93 193 98 143 
Female 46 IV — Elutriation 92 92 88 63 
Patient no.SexAge, yStage*Distant metastasis*DC generationDEC-205, %MFI DEC-205§CD83, %MFI CD83§
Female 73 IV M1a Adherence 97 210 96 91 
Male 52 IV M1c Elutriation 98 244 97 50 
Female 65 IIIc — Adherence 96 238 93 59 
Male 66 IV M1b Adherence 93 193 98 143 
Female 46 IV — Elutriation 92 92 88 63 

DC indicates dendritic cell; MFI, mean fluorescence intensity; and —, no distant metastasis.

*

According to Balch et al.35 

DC generation from monocytes enriched by the Elutra counterflow elutriation system or the standard gradient centrifugation/adherence technique.

Percentage of DEC-205-positive or CD83-positive DCs.

§

MFI above background.

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