Maturation status of DCs derived from patients with malignant melanoma
Patient no. . | Sex . | Age, y . | Stage* . | Distant metastasis* . | DC generation† . | DEC-205, %‡ . | MFI DEC-205§ . | CD83, %‡ . | MFI CD83§ . |
---|---|---|---|---|---|---|---|---|---|
1 | Female | 73 | IV | M1a | Adherence | 97 | 210 | 96 | 91 |
2 | Male | 52 | IV | M1c | Elutriation | 98 | 244 | 97 | 50 |
3 | Female | 65 | IIIc | — | Adherence | 96 | 238 | 93 | 59 |
4 | Male | 66 | IV | M1b | Adherence | 93 | 193 | 98 | 143 |
5 | Female | 46 | IV | — | Elutriation | 92 | 92 | 88 | 63 |
Patient no. . | Sex . | Age, y . | Stage* . | Distant metastasis* . | DC generation† . | DEC-205, %‡ . | MFI DEC-205§ . | CD83, %‡ . | MFI CD83§ . |
---|---|---|---|---|---|---|---|---|---|
1 | Female | 73 | IV | M1a | Adherence | 97 | 210 | 96 | 91 |
2 | Male | 52 | IV | M1c | Elutriation | 98 | 244 | 97 | 50 |
3 | Female | 65 | IIIc | — | Adherence | 96 | 238 | 93 | 59 |
4 | Male | 66 | IV | M1b | Adherence | 93 | 193 | 98 | 143 |
5 | Female | 46 | IV | — | Elutriation | 92 | 92 | 88 | 63 |
DC indicates dendritic cell; MFI, mean fluorescence intensity; and —, no distant metastasis.
According to Balch et al.35
DC generation from monocytes enriched by the Elutra counterflow elutriation system or the standard gradient centrifugation/adherence technique.
Percentage of DEC-205-positive or CD83-positive DCs.
MFI above background.