Table 2 Chromatin-modifying enzymes... | American Society of Hematology

Table 2

Chromatin-modifying enzymes essential for hematopoiesis

Enzyme	Epigenetic activity	Targeting strategy	Role in HSC self-renewal	Lineage-specific role	Ref no.
Moz	Histone acetylation	KO	Yes	No	55
		KO	Yes	Myeloid	56
Mll1	Histone methylation	KO	Yes	Myeloid	67
		KO	Yes	No	68
		CKO; Mx1-Cre,lysozyme M-Cre,CD19-Cre, lck-Cre	Yes	No	69
Ezh2	Histone methylation	CKO; Mx1-Cre	—	Lymphoid	75
		CKO; Tie2-Cre,Rosa26-Cre	No	Erythroid and lymphoid	74
Dot1L	Histone methylation	Inducible KO;Rosa26-Cre-ER	Yes	MPPs and myeloid	91
		Inducible KO;Rosa26-Cre-ER	Yes	MPPs and myeloid	90
		KO	—	Erythroid	89
Lsd1	Histone demethylation	CKD; global	Yes	Myeloid	99
Dnmt1	DNA 5mC	CKO; Mx1-Cre	Yes	Myeloid	26
		CKO; Mx1-CreHypomorph	Yes	Lymphoid	27
Dnmt3a	DNA 5mC	CKO; Mx1-Cre	Yes	Long-term HSCs	29
Tet2	DNA 5hmC	KO	Yes	Myeloid	37
		KO	Yes	Myeloid	36
		CKO; Vav-Cre,Ella-Cre, Mx1-Cre	Yes	Myeloid	38
		KOCKO; Mx1-Cre	Yes	Lymphoid and myeloid	39

Enzyme	Epigenetic activity	Targeting strategy	Role in HSC self-renewal	Lineage-specific role	Ref no.
Moz	Histone acetylation	KO	Yes	No	55
		KO	Yes	Myeloid	56
Mll1	Histone methylation	KO	Yes	Myeloid	67
		KO	Yes	No	68
		CKO; Mx1-Cre,lysozyme M-Cre,CD19-Cre, lck-Cre	Yes	No	69
Ezh2	Histone methylation	CKO; Mx1-Cre	—	Lymphoid	75
		CKO; Tie2-Cre,Rosa26-Cre	No	Erythroid and lymphoid	74
Dot1L	Histone methylation	Inducible KO;Rosa26-Cre-ER	Yes	MPPs and myeloid	91
		Inducible KO;Rosa26-Cre-ER	Yes	MPPs and myeloid	90
		KO	—	Erythroid	89
Lsd1	Histone demethylation	CKD; global	Yes	Myeloid	99
Dnmt1	DNA 5mC	CKO; Mx1-Cre	Yes	Myeloid	26
		CKO; Mx1-CreHypomorph	Yes	Lymphoid	27
Dnmt3a	DNA 5mC	CKO; Mx1-Cre	Yes	Long-term HSCs	29
Tet2	DNA 5hmC	KO	Yes	Myeloid	37
		KO	Yes	Myeloid	36
		CKO; Vav-Cre,Ella-Cre, Mx1-Cre	Yes	Myeloid	38
		KOCKO; Mx1-Cre	Yes	Lymphoid and myeloid	39

CKO, conditional knockout; CKD, conditional knockdown; 5hmC, 5-hydroxymethyl cytosine; MPP, multipotent progenitor.