Effects on rolling neutrophils in cremaster muscles
| . | Rolling cells/min . | Velocity of rolling cells, μm/s . | ||||||
|---|---|---|---|---|---|---|---|---|
| 0 min . | 30 min . | 60 min . | 90 min . | 0 min . | 30 min . | 60 min . | 90 min . | |
| MIP-2 SF | ||||||||
| WT (n = 7) | 41 ± 11 | 22 ± 6 | 16 ± 4 | 14 ± 4* | 41 ± 11 | 34 ± 6 | 31 ± 5 | 31 ± 6 |
| hpa-tg (n = 7) | 49 ± 7 | 31 ± 5 | 22 ± 4* | 17 ± 3* | 42 ± 7 | 37 ± 7 | 38 ± 8 | 31 ± 5 |
| WT + heparin (n = 5) | 40 ± 8 | 35 ± 9 | 35 ± 9 | 35 ± 9† | 39 ± 5 | 49 ± 6 | 48 ± 6† | 54 ± 5† |
| MIP-2 gel | ||||||||
| WT (n = 5) | 50 ± 9 | 30 ± 5 | 23 ± 4* | 19 ± 4* | 23 ± 4 | 25 ± 5 | 21 ± 3 | 21 ± 3 |
| . | Rolling cells/min . | Velocity of rolling cells, μm/s . | ||||||
|---|---|---|---|---|---|---|---|---|
| 0 min . | 30 min . | 60 min . | 90 min . | 0 min . | 30 min . | 60 min . | 90 min . | |
| MIP-2 SF | ||||||||
| WT (n = 7) | 41 ± 11 | 22 ± 6 | 16 ± 4 | 14 ± 4* | 41 ± 11 | 34 ± 6 | 31 ± 5 | 31 ± 6 |
| hpa-tg (n = 7) | 49 ± 7 | 31 ± 5 | 22 ± 4* | 17 ± 3* | 42 ± 7 | 37 ± 7 | 38 ± 8 | 31 ± 5 |
| WT + heparin (n = 5) | 40 ± 8 | 35 ± 9 | 35 ± 9 | 35 ± 9† | 39 ± 5 | 49 ± 6 | 48 ± 6† | 54 ± 5† |
| MIP-2 gel | ||||||||
| WT (n = 5) | 50 ± 9 | 30 ± 5 | 23 ± 4* | 19 ± 4* | 23 ± 4 | 25 ± 5 | 21 ± 3 | 21 ± 3 |
WT, hpa-tg, or WT mice pretreated with heparin (close intra-arterially, 0.5 mg/mouse) were monitored during MIP-2 superfusion (0.5nM) or after placement of an MIP-2–containing gel (0.5μM) at a distance of 400 μm from the observed venule. Values are mean ± SEM.
P < .05 vs t = 0.
P < .05 vs WT activated by MIP-2 superfusion.