hey2 expression is induced downstream of clo and scl/tal1 in the PLM and is maintained by Hh and Vegf signaling in the DA
| Mutant/morphant/treatment . | hey2 expression . | |||||
|---|---|---|---|---|---|---|
| PLM (10s) . | ALM (10s) . | Otic vesicle (10s) . | Tail bud (10s) . | DA (24-25 hpf) . | Telencephalon (24 hpf) . | |
| wt | 21/21 | 21/21 | 21/21 | 21/21 | 23/23 | 23/23 |
| cloche | 0/6 | 0/6 | 6/6 | 6/6 | 0/7 | 7/7 |
| Uninjected | 50/51 | 50/51 | 21/23 | 21/23 | 30/30 | 30/30 |
| scl/tal1 MO, 5 ng | 6/54 | 53/54 | 19/23 | 19/23 | 1/35 | 35/35 |
| Uninjected | 30/30 | 30/30 | 29/30 | 30/30 | 28/28 | 28/28 |
| etsrp MO, 10 ng | 30/31 | 1/31 | 29/31 | 31/31 | 0/26 | 26/26 |
| DMSO, 0.82% | 8/10 | 10/10 | 9/10 | 10/10 | 10/10 | 10/10 |
| Cyclopamine, 100μM | 7/10 | 7/10 | 9/10 | 10/10 | 0/23 | 23/23 |
| VegfR inhibitor, 5μM | ND | ND | ND | ND | 0/15 | 15/15 |
| wt | 24/24 | 24/24 | 23/24 | 24/24 | 27/27 | 27/27 |
| mib | 8/8 | 8/8 | 0/8 | 0/8 | 8/8 | 0/8 |
| Uninjected | 30/31 | 31/31 | 28/31 | 31/31 | 30/30 | 30/30 |
| rbpja/b MO, 2 ng | 30/31 | 31/31 | 1/31 | 0/31 | 30/30 | 1/30 |
| Mutant/morphant/treatment . | hey2 expression . | |||||
|---|---|---|---|---|---|---|
| PLM (10s) . | ALM (10s) . | Otic vesicle (10s) . | Tail bud (10s) . | DA (24-25 hpf) . | Telencephalon (24 hpf) . | |
| wt | 21/21 | 21/21 | 21/21 | 21/21 | 23/23 | 23/23 |
| cloche | 0/6 | 0/6 | 6/6 | 6/6 | 0/7 | 7/7 |
| Uninjected | 50/51 | 50/51 | 21/23 | 21/23 | 30/30 | 30/30 |
| scl/tal1 MO, 5 ng | 6/54 | 53/54 | 19/23 | 19/23 | 1/35 | 35/35 |
| Uninjected | 30/30 | 30/30 | 29/30 | 30/30 | 28/28 | 28/28 |
| etsrp MO, 10 ng | 30/31 | 1/31 | 29/31 | 31/31 | 0/26 | 26/26 |
| DMSO, 0.82% | 8/10 | 10/10 | 9/10 | 10/10 | 10/10 | 10/10 |
| Cyclopamine, 100μM | 7/10 | 7/10 | 9/10 | 10/10 | 0/23 | 23/23 |
| VegfR inhibitor, 5μM | ND | ND | ND | ND | 0/15 | 15/15 |
| wt | 24/24 | 24/24 | 23/24 | 24/24 | 27/27 | 27/27 |
| mib | 8/8 | 8/8 | 0/8 | 0/8 | 8/8 | 0/8 |
| Uninjected | 30/31 | 31/31 | 28/31 | 31/31 | 30/30 | 30/30 |
| rbpja/b MO, 2 ng | 30/31 | 31/31 | 1/31 | 0/31 | 30/30 | 1/30 |
The number of embryos with hey2 expression in a particular tissue at a particular stage is given as a fraction of the number of embryos analyzed.
ND indicates not determined.