PCR primers used for DNA analysis and mRNA analysis
| Gene . | Primer sequence . | Fragment size, bp . |
|---|---|---|
| Cre | Forward: 5′-CCGGGCTGCCACGACCA-3′ | 450 |
| Reverse: 5′-GGCGCCGCAACACCATTTTT-3′ | ||
| Slc4a1 (+ control) | Forward: 5′-CAATGGGAAACTCAATCCAAATAG-3′ | 224 |
| Reverse: 5′-TCCATCCAGCCCTTCCTTC-3′ | ||
| Neo (unexcised) | Forward: 5′-GGACCTTGCACAGATAGCGT-3′ | |
| Reverse: 5′-CTGTTCCTGACCTTGATGTG-3′ | 500 | |
| β-actin/RPS19 (excised) | Forward: 5′-GTTCGGCTTCTGGCGTGT-3′ | 226 |
| Reverse: 5′-CACGTCTTTTACAGTAACTCCAGGC-3′ | ||
| mRNA | ||
| β-actin/RPS19 | Forward: 5′-GTTCGGCTTCTGGCGTGT-3′ | |
| Reverse: 5′-CACGTCTTTTACAGTAACTCCAGGC-3′ | ||
| β2-microglobulin | Forward: 5′-TGCTATCCAGAAAACCCCTC-3′ | |
| Reverse: 5′-GTCATGCTTAACTCTGCAGG-3′ | ||
| Oligonucleotide probes for Northern blot | ||
| ITS1 | 5′-CGCCGCCGCTCCTCCACAGTCTCCCGT-3′ | |
| ITS2b | 5′-GGGTTGGCCCTGCGAGCAAACTCCC-3′ | |
| ITS2 d/e | 5′-TCTCATCGGCGCACGGACGCCGCGGCGT-3′ | |
| 18s | 5′-TTTACTTCCTCTAGATAGTCAAGTTGCACC-3′ | |
| 28s | 5′-CCCGTTCCCTTGGCTGTGGTTTCGCTGGATT-3′ |
| Gene . | Primer sequence . | Fragment size, bp . |
|---|---|---|
| Cre | Forward: 5′-CCGGGCTGCCACGACCA-3′ | 450 |
| Reverse: 5′-GGCGCCGCAACACCATTTTT-3′ | ||
| Slc4a1 (+ control) | Forward: 5′-CAATGGGAAACTCAATCCAAATAG-3′ | 224 |
| Reverse: 5′-TCCATCCAGCCCTTCCTTC-3′ | ||
| Neo (unexcised) | Forward: 5′-GGACCTTGCACAGATAGCGT-3′ | |
| Reverse: 5′-CTGTTCCTGACCTTGATGTG-3′ | 500 | |
| β-actin/RPS19 (excised) | Forward: 5′-GTTCGGCTTCTGGCGTGT-3′ | 226 |
| Reverse: 5′-CACGTCTTTTACAGTAACTCCAGGC-3′ | ||
| mRNA | ||
| β-actin/RPS19 | Forward: 5′-GTTCGGCTTCTGGCGTGT-3′ | |
| Reverse: 5′-CACGTCTTTTACAGTAACTCCAGGC-3′ | ||
| β2-microglobulin | Forward: 5′-TGCTATCCAGAAAACCCCTC-3′ | |
| Reverse: 5′-GTCATGCTTAACTCTGCAGG-3′ | ||
| Oligonucleotide probes for Northern blot | ||
| ITS1 | 5′-CGCCGCCGCTCCTCCACAGTCTCCCGT-3′ | |
| ITS2b | 5′-GGGTTGGCCCTGCGAGCAAACTCCC-3′ | |
| ITS2 d/e | 5′-TCTCATCGGCGCACGGACGCCGCGGCGT-3′ | |
| 18s | 5′-TTTACTTCCTCTAGATAGTCAAGTTGCACC-3′ | |
| 28s | 5′-CCCGTTCCCTTGGCTGTGGTTTCGCTGGATT-3′ |