Specific activity of FVIII variants before and after conjugation with 60-kDa PEG
| Sample ID . | Domain(s) of PEGylation . | Specific activity, IU/mg . | |
|---|---|---|---|
| Before PEGylation . | After PEGylation . | ||
| BDD FVIII | N/A | 8855 | N/A |
| F129C | A1 | 10521 | 9863 |
| L491C | A2 | 9428 | 8804 |
| K1804C | A3 | 9357 | 9717 |
| K1808C | A3 | 9754 | 11560 |
| Q2091 | C1 | 10546 | 4343 |
| L491C/K1804C | A2 + A3 | 8184 | 9499 |
| F129C/K1804C | A1 + A3 | 10695 | 6085 |
| Sample ID . | Domain(s) of PEGylation . | Specific activity, IU/mg . | |
|---|---|---|---|
| Before PEGylation . | After PEGylation . | ||
| BDD FVIII | N/A | 8855 | N/A |
| F129C | A1 | 10521 | 9863 |
| L491C | A2 | 9428 | 8804 |
| K1804C | A3 | 9357 | 9717 |
| K1808C | A3 | 9754 | 11560 |
| Q2091 | C1 | 10546 | 4343 |
| L491C/K1804C | A2 + A3 | 8184 | 9499 |
| F129C/K1804C | A1 + A3 | 10695 | 6085 |
The activity of non-PEGylated and PEGylated FVIII were measured by a chromogenic assay, and the protein concentration was determined by A280 absorbance based on extinction coefficient.
N/A indicates not applicable.