Table 4

HLA genotyping and microchimerism results for 4 families in which the proband was testable for 3 distinct sources of microchimerism (maternal, fetal from first delivery, and fetal from second delivery) at a single sampling time point

FamilyFamily member/Source of McHLA genotypeMicrochimerism
Sample 1Sample 2††
Proband DRB1*03 DRB1*13   
 Mother of the proband DRB1*04†† DRB1*13  
 Fetal 1 DRB1*03 DRB1*08§  
 Fetal 2 DRB1*07§ DRB1*13 22.9  
Proband DRB1*13 DRB1*12   
 Mother of the proband DRB1*13 DRB1*16§  
 Fetal 1 DRB1*04§ DRB1*12  
 Fetal 2 DRB1*01§ DRB1*12  
Proband DRB1*04 DRB1*15   
 Mother of the proband DRB1*04 DRB1*01§ 
 Fetal 1 DRB1*10§ DRB1*15 
 Fetal 2 DRB1*04 DRB1*07§ 
Proband DQB1*03 DQB1*03   
 Mother of the proband DQB1*03 DQB1*02§  
 Fetal 1 DQB1*03 DQB1*04§  
 Fetal 2 DQB1*03 DQB1*06§ 9.7  
FamilyFamily member/Source of McHLA genotypeMicrochimerism
Sample 1Sample 2††
Proband DRB1*03 DRB1*13   
 Mother of the proband DRB1*04†† DRB1*13  
 Fetal 1 DRB1*03 DRB1*08§  
 Fetal 2 DRB1*07§ DRB1*13 22.9  
Proband DRB1*13 DRB1*12   
 Mother of the proband DRB1*13 DRB1*16§  
 Fetal 1 DRB1*04§ DRB1*12  
 Fetal 2 DRB1*01§ DRB1*12  
Proband DRB1*04 DRB1*15   
 Mother of the proband DRB1*04 DRB1*01§ 
 Fetal 1 DRB1*10§ DRB1*15 
 Fetal 2 DRB1*04 DRB1*07§ 
Proband DQB1*03 DQB1*03   
 Mother of the proband DQB1*03 DQB1*02§  
 Fetal 1 DQB1*03 DQB1*04§  
 Fetal 2 DQB1*03 DQB1*06§ 9.7  

Microchimerism genome equivalents per million proband cells.

††

Samples from two different draw dates were tested for Subject 3.

§

The appropriate HLA-specific quantitative PCR assay was employed to quantify microchimerism from each source.

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