XmAbCD40 retains high affinity for CD40 antigen
| . | Biacore analysis*Kd, nM . | Cell-based assay† EC50, nM . |
|---|---|---|
| S2C6 | 0.44 ± 0.02 | 0.67 ± 0.01 |
| Chimeric‡ | 0.30 ± 0.01 | 0.56 ± 0.01 |
| XmAbCD40 | 0.47 ± 0.01 | 0.36 ± 0.01 |
| Anti-CD40 IgG1 | 0.46 ± 0.01 | 0.50 ± 0.01 |
| Anti-CD40 Fc-KO | 0.40 ± 0.01 | 0.52 ± 0.02 |
| . | Biacore analysis*Kd, nM . | Cell-based assay† EC50, nM . |
|---|---|---|
| S2C6 | 0.44 ± 0.02 | 0.67 ± 0.01 |
| Chimeric‡ | 0.30 ± 0.01 | 0.56 ± 0.01 |
| XmAbCD40 | 0.47 ± 0.01 | 0.36 ± 0.01 |
| Anti-CD40 IgG1 | 0.46 ± 0.01 | 0.50 ± 0.01 |
| Anti-CD40 Fc-KO | 0.40 ± 0.01 | 0.52 ± 0.02 |
Equilibrium dissociation constants (Kd) were obtained from Langmuir fits of the Biacore data. Results are from duplicate experiments and are mean ± SD.
Raji cell-based binding assay was used to estimate CD40 binding affinity. Antibodies were detected using fluorescently labeled secondary antibodies on a FACSCanto II flow cytometer.
Murine Fv (S2C6) and human Fc (wild-type IgG1).