Catalytic efficiencies (kcat/Km) of VWF115 proteolysis by wild-type ADAMTS13 and ADAMTS13 VR1 variants derived from time-course reactions
| . | Time course, kcat/Km, ×105 M−1s−1 . | Fold reduction . | P . |
|---|---|---|---|
| WT | 12.5 ± 3.92 | ||
| D187A | 1.27 ± 0.38 | 10 | 0.004 |
| R190A | 5.70 ± 0.42 | 2 | 0.051 |
| R193A | 3.03 ± 0.40 | 4 | 0.007 |
| VR1-AD1 | 0 | ∞ | |
| VR1-AD2 | n.d. | >25 |
| . | Time course, kcat/Km, ×105 M−1s−1 . | Fold reduction . | P . |
|---|---|---|---|
| WT | 12.5 ± 3.92 | ||
| D187A | 1.27 ± 0.38 | 10 | 0.004 |
| R190A | 5.70 ± 0.42 | 2 | 0.051 |
| R193A | 3.03 ± 0.40 | 4 | 0.007 |
| VR1-AD1 | 0 | ∞ | |
| VR1-AD2 | n.d. | >25 |
The fold reduction for each variant and the P value derived from an unpaired, 1-tailed t test is given (n = 3, except R190A for which n = 2).
WT indicates wild type; and n.d., not determined.