Catalytic efficiencies (kcat/Km) of VWF115 and VWF115(1606A) proteolysis by wild-type ADAMTS13 and ADAMTS13 VR3B variants derived from time course reactions (n=3)
| . | Time course, kcat/Km, ×105 M−1s−1) . | Fold reduction . | |
|---|---|---|---|
| VWF115 . | VWF115 (M1606A) . | ||
| WT | 7.2 ± 2.9 | 0.4 ± 0.1 | 18 |
| VR3B-AD1 | 11.9 ± 4.9 | 11.5 ± 4.2 | 0 |
| VR3B(I)-AD1 | 4.3 ± 0.7 | 4.0 ± 1.2 | 0 |
| VR3B(II)-AD1 | 7.8 ± 3.4 | 0.7 ± 0.3 | 11 |
| . | Time course, kcat/Km, ×105 M−1s−1) . | Fold reduction . | |
|---|---|---|---|
| VWF115 . | VWF115 (M1606A) . | ||
| WT | 7.2 ± 2.9 | 0.4 ± 0.1 | 18 |
| VR3B-AD1 | 11.9 ± 4.9 | 11.5 ± 4.2 | 0 |
| VR3B(I)-AD1 | 4.3 ± 0.7 | 4.0 ± 1.2 | 0 |
| VR3B(II)-AD1 | 7.8 ± 3.4 | 0.7 ± 0.3 | 11 |
WT indicates wild type.