Hematopoietic composition of young Mcl-1 transgenic mice
| Organ/cell type . | C57BL/6J . | Mcl-1(33) . | Mcl-1(4) . |
|---|---|---|---|
| Peripheral blood | 7.9 ± 3.5 | 23.6 ± 7.6* | 17.2 ± 3.6* |
| CD4+CD8− | 1.4 ± 0.7 | 3.2 ± 1.3† | 3.2 ± 1.4‡ |
| CD4−CD8+ | 0.9 ± 0.5 | 2.8 ± 0.7* | 2.1 ± 0.5† |
| B220+ IgM/IgD− | 0.6 ± 0.8 | 1.1 ± 2.1 | 0.8 ± 1.4 |
| B220+ IgM/IgD+ | 3.7 ± 2.0 | 13.7 ± 5.6* | 8.5 ± 3.0† |
| Mac1+ | 0.5 ± 0.3 | 1.5 ± 0.9‡ | 1.5 ± 1.1‡ |
| Mac1+ Gr1+ | 0.5 ± 0.4 | 0.8 ± 0.3 | 1.0 ± 0.6 |
| Spleen | 107.4 ± 40.8 | 236.9 ± 63.7* | 206.9 ± 102‡ |
| CD4+CD8− | 14.1 ± 3.4 | 42.5 ± 14.0* | 35.3 ± 16.6† |
| CD4−CD8+ | 7.0 ± 3.4 | 20.6 ± 8.3* | 17.0 ± 8.3† |
| B220+ IgM/IgD− | 1.6 ± 0.9 | 5.2 ± 2.4† | 4.5 ± 3.0‡ |
| B220+ IgM/IgD+ | 37.7 ± 17.5 | 124.1 ± 45.3* | 102.0 ± 77.1‡ |
| Mac1+ | 2.8 ± 1.8 | 5.1 ± 2.7 | 6.2 ± 3.9‡ |
| Mac1+Gr1+ | 2.0 ± 1.9 | 3.4 ± 1.6 | 3.6 ± 1.3 |
| Ter-119+ | 29.8 ± 25.9 | 40.4 ± 12 | 32.9 ± 23.5 |
| LN | 12.4 ± 5.1 | 25.8 ± 7.5† | 20 ± 8.7 |
| CD4+CD8− | 4.4 ± 1.7 | 9.7 ± 3.1† | 7.9 ± 3.0‡ |
| CD4−CD8+ | 3.0 ± 1.1 | 6.5 ± 2.0†‡ | 5.3 ± 2.6 |
| B220+ IgM/IgD | 0.14 ± 0.05 | 0.30 ± 0.17‡ | 0.29 ± 0.16 |
| B220+ IgM/IgD+ | 4.0 ± 1.4 | 8.2 ± 2.3† | 4.9 ± 2.1 |
| BM | 25.0 ± 5.4 | 26.0 ± 7.3 | 27.7 ± 10.6 |
| Ter 119+ | 10.8 ± 3.9 | 6.0 ± 4.7 | 12.0 ± 6.5 |
| Thy1+ | 1.7 ± 1.7 | 1.5 ± 0.6 | 1.3 ± 0.5 |
| B220+ IgM/IgD | 3.5 ± 2.2 | 5.6 ± 2.4 | 4.2 ± 2.0 |
| B220+ IgM/IgD+ | 2.0 ± 1.3 | 4.9 ± 2.1† | 3.4 ± 1.7 |
| Mac1+ | 1.5 ± 1.7 | 2.5 ± 1.4 | 2.0 ± 1.9 |
| Mac1+Gr1+ | 5.5 ± 4.9 | 5.2 ± 3.2 | 4.5 ± 2.4 |
| Thymus | 170.6 ± 76.4 | 1509 ± 70.7 | 193.9 ± 55.0 |
| CD4−CD8− | 9.6 ± 5.5 | 7.2 ± 3.9 | 9.6 ± 5.3 |
| CD4+CD8+ | 141.6 ± 64.8 | 123.6 ± 62.1 | 163.0 ± 49.0 |
| CD4+CD8− | 12.7 ± 5.3 | 14.2 ± 6.3 | 13.4 ± 3.5 |
| CD4−CD8+ | 6.7 ± 4.1 | 5.9 ± 3.0 | 7.9 ± 2.6 |
| Organ/cell type . | C57BL/6J . | Mcl-1(33) . | Mcl-1(4) . |
|---|---|---|---|
| Peripheral blood | 7.9 ± 3.5 | 23.6 ± 7.6* | 17.2 ± 3.6* |
| CD4+CD8− | 1.4 ± 0.7 | 3.2 ± 1.3† | 3.2 ± 1.4‡ |
| CD4−CD8+ | 0.9 ± 0.5 | 2.8 ± 0.7* | 2.1 ± 0.5† |
| B220+ IgM/IgD− | 0.6 ± 0.8 | 1.1 ± 2.1 | 0.8 ± 1.4 |
| B220+ IgM/IgD+ | 3.7 ± 2.0 | 13.7 ± 5.6* | 8.5 ± 3.0† |
| Mac1+ | 0.5 ± 0.3 | 1.5 ± 0.9‡ | 1.5 ± 1.1‡ |
| Mac1+ Gr1+ | 0.5 ± 0.4 | 0.8 ± 0.3 | 1.0 ± 0.6 |
| Spleen | 107.4 ± 40.8 | 236.9 ± 63.7* | 206.9 ± 102‡ |
| CD4+CD8− | 14.1 ± 3.4 | 42.5 ± 14.0* | 35.3 ± 16.6† |
| CD4−CD8+ | 7.0 ± 3.4 | 20.6 ± 8.3* | 17.0 ± 8.3† |
| B220+ IgM/IgD− | 1.6 ± 0.9 | 5.2 ± 2.4† | 4.5 ± 3.0‡ |
| B220+ IgM/IgD+ | 37.7 ± 17.5 | 124.1 ± 45.3* | 102.0 ± 77.1‡ |
| Mac1+ | 2.8 ± 1.8 | 5.1 ± 2.7 | 6.2 ± 3.9‡ |
| Mac1+Gr1+ | 2.0 ± 1.9 | 3.4 ± 1.6 | 3.6 ± 1.3 |
| Ter-119+ | 29.8 ± 25.9 | 40.4 ± 12 | 32.9 ± 23.5 |
| LN | 12.4 ± 5.1 | 25.8 ± 7.5† | 20 ± 8.7 |
| CD4+CD8− | 4.4 ± 1.7 | 9.7 ± 3.1† | 7.9 ± 3.0‡ |
| CD4−CD8+ | 3.0 ± 1.1 | 6.5 ± 2.0†‡ | 5.3 ± 2.6 |
| B220+ IgM/IgD | 0.14 ± 0.05 | 0.30 ± 0.17‡ | 0.29 ± 0.16 |
| B220+ IgM/IgD+ | 4.0 ± 1.4 | 8.2 ± 2.3† | 4.9 ± 2.1 |
| BM | 25.0 ± 5.4 | 26.0 ± 7.3 | 27.7 ± 10.6 |
| Ter 119+ | 10.8 ± 3.9 | 6.0 ± 4.7 | 12.0 ± 6.5 |
| Thy1+ | 1.7 ± 1.7 | 1.5 ± 0.6 | 1.3 ± 0.5 |
| B220+ IgM/IgD | 3.5 ± 2.2 | 5.6 ± 2.4 | 4.2 ± 2.0 |
| B220+ IgM/IgD+ | 2.0 ± 1.3 | 4.9 ± 2.1† | 3.4 ± 1.7 |
| Mac1+ | 1.5 ± 1.7 | 2.5 ± 1.4 | 2.0 ± 1.9 |
| Mac1+Gr1+ | 5.5 ± 4.9 | 5.2 ± 3.2 | 4.5 ± 2.4 |
| Thymus | 170.6 ± 76.4 | 1509 ± 70.7 | 193.9 ± 55.0 |
| CD4−CD8− | 9.6 ± 5.5 | 7.2 ± 3.9 | 9.6 ± 5.3 |
| CD4+CD8+ | 141.6 ± 64.8 | 123.6 ± 62.1 | 163.0 ± 49.0 |
| CD4+CD8− | 12.7 ± 5.3 | 14.2 ± 6.3 | 13.4 ± 3.5 |
| CD4−CD8+ | 6.7 ± 4.1 | 5.9 ± 3.0 | 7.9 ± 2.6 |
Nucleated cells × 106, except peripheral blood cells (× 106/mL). Nucleated cells (mean ± SD) from 6- to 8-week-old male mice; n = 4 to 9 mice per genotype.
Significantly different from WT (P ≤ .001, Student t test).
Significantly different from WT (P ≤ .01, Student t test).
Significantly different from WT (P ≤ .05, Student t test).