The numbers in the table refer to the number of unique sequences, where only 1 example of a clonal expansion is counted, the overall total is the total number of sequences obtained before each adjustment was made. There is a high rate of attrition of sequence reads from the 454. The numbers here represent, on average, 20% of the initial data as 60% of sequences either do not pass our quality control checks or do not return results from V-QUEST.²⁴  A further 20% were removed as they lacked some component of the full immunoglobulin VDJC gene rearrangement, for example, some of the sequences in the IgD-CD27- or IgD- CD27+ populations could not be identified as being switched due to lack of C region sequence so were not included.