Primers and probes used in qRT-PCR analyses
| . | Primers and probes . |
|---|---|
| Osteocalcin | Forward (32f) 5′-TTCTGCTCACTCTGCTGACCCT-3′ |
| Reverse (137b) 5′-CCCTCCTGCTTGGACATGAA-3′ | |
| Osterix | Forward (126f) 5′-AGCTCACTATGGCTCCAGTCC-3′ |
| Reverse (245b) 5′-GCGTATGGCTTCTTTGTGCCT-3′ | |
| CXCL12 | Forward (198f) 5′-GAGCCAACGTCAAGCATCTG-3′ |
| Reverse (298b) 5′-CGGGTCAATGCACACTTGTC-3′ | |
| KL | Forward (80f) 5′-GCTACCCAATGCTGGGACTA-3′ |
| Reverse (286b) 5′-CCGCAGATCTCCTTGGTTT-3′ | |
| Angiopoietin-1 | Forward (1645f) 5′-CAAATGCGCTCTCATGCTAA-3′ |
| Reverse (1806b) 5′-ATGGTGGTGGAACGTAAGGA-3′ | |
| VE-cadherin | Forward (722f) 5′-CGTGGTGGAAACACAAGATG-3′ |
| Reverse (902b) 5′-TGGGTCCACAACAGTCAGAA-3′ | |
| Fibronectin | Forward (51f) 5′-GTCAGTCAAAGCAAGCCCGG-3′ |
| Reverse (288b) 5′-CAGTCCCAGATCATGGAGTC-3′ | |
| Runx2 | Forward (904f) 5′-CCAAGAAGGCACAGACAGAA-3′ |
| Reverse (995b) 5′-ATACTGGGATGAGGAATGCG-3′ | |
| Probe(949b)FAM-5′-CCCTAAATCACTGAGGCGATCAGAGAA-3′-BHQ1 | |
| PTHR1 | Forward(894f) 5′-GATTCTGGTGGAGGGACTGT |
| Reverse(1090b) 5′-GGATGATCCACTTCTTGTGC-3′ | |
| Probe(1039b)FAM-5′-TCCCAGCACCCAGTGTTGGC-3′-BHQ1 | |
| β2-microglobulin | Forward 5′-CTGGTCTTTCTGGTGCTTGTC-3′ |
| Reverse 5′-GTATGTTCGGCTTCCCATTC-3′ | |
| probe(94f)FAM-5′-CACTGACCGGCCTGTATGCTATCCA-3′-BHQ1 |
| . | Primers and probes . |
|---|---|
| Osteocalcin | Forward (32f) 5′-TTCTGCTCACTCTGCTGACCCT-3′ |
| Reverse (137b) 5′-CCCTCCTGCTTGGACATGAA-3′ | |
| Osterix | Forward (126f) 5′-AGCTCACTATGGCTCCAGTCC-3′ |
| Reverse (245b) 5′-GCGTATGGCTTCTTTGTGCCT-3′ | |
| CXCL12 | Forward (198f) 5′-GAGCCAACGTCAAGCATCTG-3′ |
| Reverse (298b) 5′-CGGGTCAATGCACACTTGTC-3′ | |
| KL | Forward (80f) 5′-GCTACCCAATGCTGGGACTA-3′ |
| Reverse (286b) 5′-CCGCAGATCTCCTTGGTTT-3′ | |
| Angiopoietin-1 | Forward (1645f) 5′-CAAATGCGCTCTCATGCTAA-3′ |
| Reverse (1806b) 5′-ATGGTGGTGGAACGTAAGGA-3′ | |
| VE-cadherin | Forward (722f) 5′-CGTGGTGGAAACACAAGATG-3′ |
| Reverse (902b) 5′-TGGGTCCACAACAGTCAGAA-3′ | |
| Fibronectin | Forward (51f) 5′-GTCAGTCAAAGCAAGCCCGG-3′ |
| Reverse (288b) 5′-CAGTCCCAGATCATGGAGTC-3′ | |
| Runx2 | Forward (904f) 5′-CCAAGAAGGCACAGACAGAA-3′ |
| Reverse (995b) 5′-ATACTGGGATGAGGAATGCG-3′ | |
| Probe(949b)FAM-5′-CCCTAAATCACTGAGGCGATCAGAGAA-3′-BHQ1 | |
| PTHR1 | Forward(894f) 5′-GATTCTGGTGGAGGGACTGT |
| Reverse(1090b) 5′-GGATGATCCACTTCTTGTGC-3′ | |
| Probe(1039b)FAM-5′-TCCCAGCACCCAGTGTTGGC-3′-BHQ1 | |
| β2-microglobulin | Forward 5′-CTGGTCTTTCTGGTGCTTGTC-3′ |
| Reverse 5′-GTATGTTCGGCTTCCCATTC-3′ | |
| probe(94f)FAM-5′-CACTGACCGGCCTGTATGCTATCCA-3′-BHQ1 |