ECP-enhanced expression of cell adhesion–associated genes ratio* of ECP Day 1/Pre-ECP levels
| Gene . | Attributes normal . | CTCL and GVHD (N = 6) induced expression ratio . | Normal subjects (N = 6) induced expression ratio . |
|---|---|---|---|
| Integrin β8 (ITGB8) | Expressed on DCs and plays a role in TGF-β activation to prevent immune dysfunction.27 | 26.3 | 4.2 |
| P = 5.0 × 10−12 | P = 1.2 × 10−5 | ||
| Integrin α5 (ITGA5) | Component of fibronectin receptor.28 | 3.1 | 3.1 |
| P = 1.3 × 10−5 | P = 7.4 × 10−5 | ||
| Integrin αV (ITGAV) | Component of vitronectin receptor.29 | 3.7 | 4.0 |
| P = 6.6 × 10−6 | P = 9.4 × 10−6 | ||
| Osteopontin (SPP1) | Regulates cytokine production in DCs.30 | 45.6 | 15.7 |
| P = 4.1 × 10−8 | P = 1.1 × 10−5 | ||
| ICAM 1 | Mediates DC interaction with T cells.31 | 3.2 | 3.7 |
| P = 1.3 × 10−5 | P = 7.2 × 10−4 | ||
| Matrix metalloprotease | |||
| MMP9 | Mature DC marker; contributes to migration.32 | 17.3 | 6.8 |
| P = 3.3 × 10−7 | P = 1.9 × 10−4 |
| Gene . | Attributes normal . | CTCL and GVHD (N = 6) induced expression ratio . | Normal subjects (N = 6) induced expression ratio . |
|---|---|---|---|
| Integrin β8 (ITGB8) | Expressed on DCs and plays a role in TGF-β activation to prevent immune dysfunction.27 | 26.3 | 4.2 |
| P = 5.0 × 10−12 | P = 1.2 × 10−5 | ||
| Integrin α5 (ITGA5) | Component of fibronectin receptor.28 | 3.1 | 3.1 |
| P = 1.3 × 10−5 | P = 7.4 × 10−5 | ||
| Integrin αV (ITGAV) | Component of vitronectin receptor.29 | 3.7 | 4.0 |
| P = 6.6 × 10−6 | P = 9.4 × 10−6 | ||
| Osteopontin (SPP1) | Regulates cytokine production in DCs.30 | 45.6 | 15.7 |
| P = 4.1 × 10−8 | P = 1.1 × 10−5 | ||
| ICAM 1 | Mediates DC interaction with T cells.31 | 3.2 | 3.7 |
| P = 1.3 × 10−5 | P = 7.2 × 10−4 | ||
| Matrix metalloprotease | |||
| MMP9 | Mature DC marker; contributes to migration.32 | 17.3 | 6.8 |
| P = 3.3 × 10−7 | P = 1.9 × 10−4 |
ECP-induced fold increase in expression of genes affecting monocyte adhesion, migration, and signaling.
Impact of treatment on gene expression is displayed as an induced expression ratio (ratio of ECP Day 1 to pre-ECP expression for the relevant gene). RNA was isolated from 3 CTCL patients and 3 GVHD patients and 6 normal subjects at the relevant time points.