Selected transcription factors significantly represented in genes in CHE and DE_SigUp with examples of functions
| TF . | TFBS enriched in . | Erythroid-related functions . |
|---|---|---|
| CREB | CHE, DE_SigUp | Key mediator of myeloid differentiation and proliferation |
| AP2/ERF | CHE | Transcriptional repressor, induces erythroid differentiation in K562 and HEL cells30 |
| E2F | CHE, DE_SigUp | E2F plays critical roles in cell cycle progression by regulating the expression of genes involved in nucleotide synthesis, DNA replication, and cell cycle control as demonstrated in mouse models.38 E2F has recently been shown to form a complex with GATA1, FOG1, and retinoblastoma protein (pRb), thus stalling cell proliferation and steering erythroid precursors toward terminal differentiation.39 |
| NFY(A) (CAAT-binding protein) | CHE, DE_SigUp | Binds human β-globin promoter in K562 cells, stabilizing a complex formed with MZF1 and GATA2. Appears important in maintaining a chromatin conformation favorable to β-globin transcription.40 |
| SP1 | CHE, DE_SigUp | Ubiquitously expressed. Acts synergistically with FOXM1, GATA1,27 OCT, and AP2, which are also highlighted in DiRE analysis of DE_SigUp. |
| TATA/TBP | DE_SigUp | TBP is essential for long-range activation of β-globin transcription in response to the HS2 enhancer located 10 kb from the promoter.41 |
| TF . | TFBS enriched in . | Erythroid-related functions . |
|---|---|---|
| CREB | CHE, DE_SigUp | Key mediator of myeloid differentiation and proliferation |
| AP2/ERF | CHE | Transcriptional repressor, induces erythroid differentiation in K562 and HEL cells30 |
| E2F | CHE, DE_SigUp | E2F plays critical roles in cell cycle progression by regulating the expression of genes involved in nucleotide synthesis, DNA replication, and cell cycle control as demonstrated in mouse models.38 E2F has recently been shown to form a complex with GATA1, FOG1, and retinoblastoma protein (pRb), thus stalling cell proliferation and steering erythroid precursors toward terminal differentiation.39 |
| NFY(A) (CAAT-binding protein) | CHE, DE_SigUp | Binds human β-globin promoter in K562 cells, stabilizing a complex formed with MZF1 and GATA2. Appears important in maintaining a chromatin conformation favorable to β-globin transcription.40 |
| SP1 | CHE, DE_SigUp | Ubiquitously expressed. Acts synergistically with FOXM1, GATA1,27 OCT, and AP2, which are also highlighted in DiRE analysis of DE_SigUp. |
| TATA/TBP | DE_SigUp | TBP is essential for long-range activation of β-globin transcription in response to the HS2 enhancer located 10 kb from the promoter.41 |