Table 1 Reconstitution of the lymphocyte... | American Society of Hematology

Table 1

Reconstitution of the lymphocyte compartment following allo-BMT

	Day 7	Day 15	Day 30	Day 60
CD4 T cell, ×10⁶
n.m.	21.92 ± 2.16*	18.56 ± 1.10*	23.229 ± 1.74*	23.97 ± 3.98*
Tallo	0.18 ± 0.06†	1.77 ± 0.38	2.86 ± 2.11
Tallo+rsTreg	0.38 ± 0.05	2.00 ± 0.78	2.95 ± 0.56	1.09 ± 0.15
CD8 T cell, ×10⁶
n.m.	15.56 ± 1.42*	12.99 ± 1.30*	16.63 ± 1.70*	17.82 ± 3.00*
Tallo	0.16 ± 0.07	2.37 ± 0.44	3.11 ± 2.26
Tallo+rsTreg	0.26 ± 0.04	3.35 ± 0.93	5.71 ± 1.30	1.63 ± 0.25
B cell, ×10⁶
n.m.	69.26 ± 5.52*	78.24 ± 3.39*	70.50 ± 7.13*	83.77 ± 12.52*
Tallo	n.d.	0.05 ± 0.01†	0.49 ± 0.25†
Tallo+rsTreg	0.04 ± 0.01	2.48 ± 1.00	15.25 ± 5.20	11.07 ± 4.39

	Day 7	Day 15	Day 30	Day 60
CD4 T cell, ×10⁶
n.m.	21.92 ± 2.16*	18.56 ± 1.10*	23.229 ± 1.74*	23.97 ± 3.98*
Tallo	0.18 ± 0.06†	1.77 ± 0.38	2.86 ± 2.11
Tallo+rsTreg	0.38 ± 0.05	2.00 ± 0.78	2.95 ± 0.56	1.09 ± 0.15
CD8 T cell, ×10⁶
n.m.	15.56 ± 1.42*	12.99 ± 1.30*	16.63 ± 1.70*	17.82 ± 3.00*
Tallo	0.16 ± 0.07	2.37 ± 0.44	3.11 ± 2.26
Tallo+rsTreg	0.26 ± 0.04	3.35 ± 0.93	5.71 ± 1.30	1.63 ± 0.25
B cell, ×10⁶
n.m.	69.26 ± 5.52*	78.24 ± 3.39*	70.50 ± 7.13*	83.77 ± 12.52*
Tallo	n.d.	0.05 ± 0.01†	0.49 ± 0.25†
Tallo+rsTreg	0.04 ± 0.01	2.48 ± 1.00	15.25 ± 5.20	11.07 ± 4.39

[BALB/c × C57BL/6]F1 recipient mice were lethally irradiated and then grafted with 3 × 10⁶ CD3ε^−/− BM cells and 3 × 10⁶ allogeneic Ly5.1⁺ C57BL/6 T cells alone (Tallo) or with 3 × 10⁶ Thy1.1⁺ rsTreg (Tallo+rsTreg). Unmanipulated mice represent the control group (n.m.). Immune reconstitution of T- (CD4⁺ and CD8⁺) and B-cell compartments was evaluated in the spleen 7, 15, 30, and 60 days after transplantation. SD is indicated for each mean value. Data were cumulated from 4 independent experiments with 2 to 5 mice per group per time point.

n.d. indicates nondetectable.

P < .001 for n.m. vs Tallo and for n.m. vs Tallo+rsTreg.

†

P < 0.05 for Tallo vs Tallo+rsTreg.

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