Primers and probes used for real-time PCR
| Name . | Sequence or TaqMan gene expression assay number* . | Modification . | Concentration . |
|---|---|---|---|
| LV vector | |||
| GAGF | 5′-GGAGCTAGAACGATTCGCAGTTA-3′ | 720nM | |
| GAGR | 5′-GGTTGTAGCTGTCCCAGTATTTGTC-3′ | 720nM | |
| GAGP1 | 5′-ACAGCCTTCTGATGTCTCTAAAAGGCCAGG-3′ | 5′FAM 3′TAMRA | 140nM |
| β-actin | |||
| mβactF1 | 5′-ACGGCCAGGTCATCACTATTG-3′ | 900nM | |
| mβactR1 | 5′-CAAGAAGGAAGGCTGGAAAAGA-3′ | 900nM | |
| mβactP1 | 5′-CAACGAGCGGTTCCGATGCCCT-3′ | 5′FAM 3′TAMRA | 250nM |
| SRY | Mm00441712_s1* | 5′FAM 3′NFQ-MGB | 1X |
| EpoR | Mm00833882_m1* | 5′FAM 3′NFQ-MGB | 1X |
| GAPDH | Mm99999915_g1* | 5′FAM 3′NFQ-MGB | 1X |
| 18s | Hs99999901_s1* | 5′FAM 3′NFQ-MGB | 1X |
| Name . | Sequence or TaqMan gene expression assay number* . | Modification . | Concentration . |
|---|---|---|---|
| LV vector | |||
| GAGF | 5′-GGAGCTAGAACGATTCGCAGTTA-3′ | 720nM | |
| GAGR | 5′-GGTTGTAGCTGTCCCAGTATTTGTC-3′ | 720nM | |
| GAGP1 | 5′-ACAGCCTTCTGATGTCTCTAAAAGGCCAGG-3′ | 5′FAM 3′TAMRA | 140nM |
| β-actin | |||
| mβactF1 | 5′-ACGGCCAGGTCATCACTATTG-3′ | 900nM | |
| mβactR1 | 5′-CAAGAAGGAAGGCTGGAAAAGA-3′ | 900nM | |
| mβactP1 | 5′-CAACGAGCGGTTCCGATGCCCT-3′ | 5′FAM 3′TAMRA | 250nM |
| SRY | Mm00441712_s1* | 5′FAM 3′NFQ-MGB | 1X |
| EpoR | Mm00833882_m1* | 5′FAM 3′NFQ-MGB | 1X |
| GAPDH | Mm99999915_g1* | 5′FAM 3′NFQ-MGB | 1X |
| 18s | Hs99999901_s1* | 5′FAM 3′NFQ-MGB | 1X |
EpoR indicates erythropoietin receptor; FAM, 6-carboxyfluorescein ester; LV, lentiviral vector; NFQ, nonfluorescent quencher; MGB, minor groove binder; SRY, sex-determining region Y and TAMRA, tetramethyl-6-carboxyrhodamine.
Applied Biosystems.