Hematopoietic cell numbers in SHIP1el20/el20 mice
| . | SHIP1+/+ . | SHIP1el20/el20 . | SHIP1−/− . |
|---|---|---|---|
| Peripheral blood | |||
| Hematocrit, % | 44.7 ± 1.0 | 40.5 ± 1.1* | 39.6 ± 4.2* |
| White cells, × 109/L | 10.9 ± 0.8 | 7.2 ± 0.2*† | 9.9 ± 0.7 |
| Lymphocytes, × 109/L | 8.8 ± 0.6 | 5.1 ± 0.2*† | 7.2 ± 0.6 |
| Neutrophils, × 109/L | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.4 |
| Platelets, × 109/L | 1016 ± 81 | 499 ± 52* | 607 ± 78* |
| Bone marrow, cells × 106 | 61 ± 4 | 49 ± 5*† | 71 ± 5 |
| Pre-B | 9.4 ± 1.7 | 0.2 ± 0.1*† | 9.0 ± 0.2 |
| Mature B | 2.5 ± 0.9 | 0.2 ± 0.1*† | 2.8 ± 0.2 |
| Mac-1+ | 18 ± 0.4 | 23 ± 3.6 | 22 ± 1.4* |
| Mac-1+Gr-1+ | 7.3 ± 0.8 | 21 ± 2.3* | NA |
| Pro-erythroblast | 23 ± 1.6 | 20 ± 4.8 | 23 ± 2.3 |
| Late erythroblast | 10 ± 1.2 | 5.7 ± 0.7* | 15 ± 0.4* |
| Spleen, cells × 106 | 107 ± 8 | 224 ± 4*† | 98 ± 14 |
| B220+ | 17 ± 5 | 9 ± 3† | 23 ± 3 |
| CD4+ | 12 ± 3 | 6 ± 1* | 9 ± 1 |
| CD8+ | 7 ± 1 | 4 ± 1* | 5 ± 1 |
| Mac-1+ | 18 ± 4 | 23 ± 01 | 14 ± 6 |
| Mac-1+Gr-1+ | 16 ± 2 | 22 ± 2* | NA |
| Pro-erythroblast | 9 ± 2 | 143 ± 26*† | 14 ± 2* |
| Late erythroblast | 24 ± 4 | 28 ± 0.7 | 19 ± 1 |
| Thymus, cells × 106 | 91 ± 13 | 6.5 ± 2.7* | 103 ± 3 |
| DN | 3.1 ± 0.6 | 1.4 ± 0.7*† | 3.1 ± 0.3 |
| DP | 76 ± 12 | 2.7 ± 0.7*† | 80 ± 10 |
| CD4+ | 8.4 ± 1.4 | 1.7 ± 0.3*† | 8.9 ± 1.1 |
| CD8+ | 2.8 ± 0.5 | 0.7 ± 0.2*† | 4.5 ± 0.5* |
| . | SHIP1+/+ . | SHIP1el20/el20 . | SHIP1−/− . |
|---|---|---|---|
| Peripheral blood | |||
| Hematocrit, % | 44.7 ± 1.0 | 40.5 ± 1.1* | 39.6 ± 4.2* |
| White cells, × 109/L | 10.9 ± 0.8 | 7.2 ± 0.2*† | 9.9 ± 0.7 |
| Lymphocytes, × 109/L | 8.8 ± 0.6 | 5.1 ± 0.2*† | 7.2 ± 0.6 |
| Neutrophils, × 109/L | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.4 |
| Platelets, × 109/L | 1016 ± 81 | 499 ± 52* | 607 ± 78* |
| Bone marrow, cells × 106 | 61 ± 4 | 49 ± 5*† | 71 ± 5 |
| Pre-B | 9.4 ± 1.7 | 0.2 ± 0.1*† | 9.0 ± 0.2 |
| Mature B | 2.5 ± 0.9 | 0.2 ± 0.1*† | 2.8 ± 0.2 |
| Mac-1+ | 18 ± 0.4 | 23 ± 3.6 | 22 ± 1.4* |
| Mac-1+Gr-1+ | 7.3 ± 0.8 | 21 ± 2.3* | NA |
| Pro-erythroblast | 23 ± 1.6 | 20 ± 4.8 | 23 ± 2.3 |
| Late erythroblast | 10 ± 1.2 | 5.7 ± 0.7* | 15 ± 0.4* |
| Spleen, cells × 106 | 107 ± 8 | 224 ± 4*† | 98 ± 14 |
| B220+ | 17 ± 5 | 9 ± 3† | 23 ± 3 |
| CD4+ | 12 ± 3 | 6 ± 1* | 9 ± 1 |
| CD8+ | 7 ± 1 | 4 ± 1* | 5 ± 1 |
| Mac-1+ | 18 ± 4 | 23 ± 01 | 14 ± 6 |
| Mac-1+Gr-1+ | 16 ± 2 | 22 ± 2* | NA |
| Pro-erythroblast | 9 ± 2 | 143 ± 26*† | 14 ± 2* |
| Late erythroblast | 24 ± 4 | 28 ± 0.7 | 19 ± 1 |
| Thymus, cells × 106 | 91 ± 13 | 6.5 ± 2.7* | 103 ± 3 |
| DN | 3.1 ± 0.6 | 1.4 ± 0.7*† | 3.1 ± 0.3 |
| DP | 76 ± 12 | 2.7 ± 0.7*† | 80 ± 10 |
| CD4+ | 8.4 ± 1.4 | 1.7 ± 0.3*† | 8.9 ± 1.1 |
| CD8+ | 2.8 ± 0.5 | 0.7 ± 0.2*† | 4.5 ± 0.5* |
Analyses of peripheral blood and hematopoietic organs were performed on 5-week-old SHIP1+/+, SHIP1−/−, and SHIP1el20/el20 mice. Values are the mean ± SD of at least 3 mice for each genotype. BM cell numbers were calculated per femur.
Pre-B indicates B220loCD25+; mature B, B220hiIgM+; Pro-erythroblast, ter119+CD71hi; Late erythroblast, ter119+CD71−; and NA, not assessed.
P < .05 by 2-tailed Mann-Whitney test versus SHIP1+/+ controls.
P < .05 by 2-tailed Mann-Whitney test versus SHIP1−/− mice.