PCR Primers used in this study
| . | Primers . |
|---|---|
| End point PCR | |
| Mouse Postn | 5′-ATTGGTACCTCCATCCAAGACT-3′ |
| 5′-TGTGTCAGGAGATCTTTCAAAT-3′ | |
| Gapdh control | 5′-TGAGTATGTCGTGGAGTCTACTGG-3′ |
| 5′-GTGGCAGTGATGGCATGGAC-3′. | |
| sqRT-PCR | |
| Rabbit Postn | 5′-CATGAACAGATCTCTTGACACAA-3′ |
| 5′-TCCAATGGGTATGTCTGCT-3′ | |
| Gapdh control | 5′-CATCACTGCCACCCAGAAGA-3′ |
| 5′-CCTGCTTCACCACCTTCTTGA-3′ | |
| qRT-PCR | |
| Mouse IL-7 | 5′-GGCAGGTTGTGCAAACCAGTAACA-3′ |
| 5′-AAAGGAAGCCTCTGTTCCCATGTGT-3′ | |
| Mouse CXCL12 | 5′-ACACTCCAAACTGTGCCCTTCAGA-3′ |
| 5′-AAGAGGCTCAAGATGTGAGAGGCT-3′ | |
| Mouse Postn | 5′-AGGCAAACAGCTCAGAGTCTTCGT-3′ |
| 5′-ATGGAGGGATTTCTCTGCTGGCTT-3′ | |
| Hgprt control | 5′-GTCAACGGGGGACATAAAAG-3′ |
| 5′-TGGGGCTGTACTGCTTAACC-3′ | |
| Postn mutagenesis primers* | 5′-GCTGCCCCGCAGTCATGCCTATA' |
| GATCATGTTTATGGCAC-3′ | |
| 5′-GTGCCATAAACATGATCTATAGG' | |
| CATGACTGCGGGGCAGC-3′ |
| . | Primers . |
|---|---|
| End point PCR | |
| Mouse Postn | 5′-ATTGGTACCTCCATCCAAGACT-3′ |
| 5′-TGTGTCAGGAGATCTTTCAAAT-3′ | |
| Gapdh control | 5′-TGAGTATGTCGTGGAGTCTACTGG-3′ |
| 5′-GTGGCAGTGATGGCATGGAC-3′. | |
| sqRT-PCR | |
| Rabbit Postn | 5′-CATGAACAGATCTCTTGACACAA-3′ |
| 5′-TCCAATGGGTATGTCTGCT-3′ | |
| Gapdh control | 5′-CATCACTGCCACCCAGAAGA-3′ |
| 5′-CCTGCTTCACCACCTTCTTGA-3′ | |
| qRT-PCR | |
| Mouse IL-7 | 5′-GGCAGGTTGTGCAAACCAGTAACA-3′ |
| 5′-AAAGGAAGCCTCTGTTCCCATGTGT-3′ | |
| Mouse CXCL12 | 5′-ACACTCCAAACTGTGCCCTTCAGA-3′ |
| 5′-AAGAGGCTCAAGATGTGAGAGGCT-3′ | |
| Mouse Postn | 5′-AGGCAAACAGCTCAGAGTCTTCGT-3′ |
| 5′-ATGGAGGGATTTCTCTGCTGGCTT-3′ | |
| Hgprt control | 5′-GTCAACGGGGGACATAAAAG-3′ |
| 5′-TGGGGCTGTACTGCTTAACC-3′ | |
| Postn mutagenesis primers* | 5′-GCTGCCCCGCAGTCATGCCTATA' |
| GATCATGTTTATGGCAC-3′ | |
| 5′-GTGCCATAAACATGATCTATAGG' | |
| CATGACTGCGGGGCAGC-3′ |
Inserted mutations are underlined.