Antibodies used in this study
| Specificity (name) . | Clonality . | Class . | Species . | Clone . | Manufacturer . | Application (fluorescence) . |
|---|---|---|---|---|---|---|
| Anti-P1 (ImmuClone) | Monoclonal | IgM | Human | P3NIL100 | Immucor | Hemagglutination, FCM/primary |
| Anti-P1 (Seraclone) | Monoclonal | IgM | Mouse | 650 | Biotest | Hemagglutination |
| Anti-P1 | Polyclonal | IgM | Goat | — | Immucor | Hemagglutination |
| Anti-CD77 | Monoclonal | IgM | Rat | 38-13 | Immunotech | FCM/primary |
| Anti–human IgM | Polyclonal | F(ab′)2 | Rabbit | — | DakoCytomation | FCM/secondary (FITC) |
| Anti–rat IgM | Polyclonal | F(ab′)2 | Goat | — | Beckman Coulter | FCM/secondary (PE) |
| Specificity (name) . | Clonality . | Class . | Species . | Clone . | Manufacturer . | Application (fluorescence) . |
|---|---|---|---|---|---|---|
| Anti-P1 (ImmuClone) | Monoclonal | IgM | Human | P3NIL100 | Immucor | Hemagglutination, FCM/primary |
| Anti-P1 (Seraclone) | Monoclonal | IgM | Mouse | 650 | Biotest | Hemagglutination |
| Anti-P1 | Polyclonal | IgM | Goat | — | Immucor | Hemagglutination |
| Anti-CD77 | Monoclonal | IgM | Rat | 38-13 | Immunotech | FCM/primary |
| Anti–human IgM | Polyclonal | F(ab′)2 | Rabbit | — | DakoCytomation | FCM/secondary (FITC) |
| Anti–rat IgM | Polyclonal | F(ab′)2 | Goat | — | Beckman Coulter | FCM/secondary (PE) |
IgM indicates immunoglobulin M; FCM, flow cytometry; FITC, fluorescein isothiocyanate; PE, phycoerythrin; and —, not applicable.