Table 1.

Correlation between peripheral-blood leukocyte expression of CD25, CD4, and 38 surface markers


Staining pattern versus CD25*for cells gated for expression of CD4

Antibodies
All cells negative or very low   CD8, CD21, CD23, CD40L, CD54, CD70§, CD74, CD84, CXCR5, CD148 
Large negative population, including all CD25+ cells   CD56, CD95L, CD244§, CXCR4 
All cells positive   CD3 
Most cells positive, including all CD25+ cells   CD5, CD11a, CD28#, CD57 
  CD27, CD38, CD44, CD45RA, CD45RO, CD58, CD62L, CD71, CD95,  
Profiles shown in Figure 2   CTLA-4 (CD152), HLA-DR, CCR7 
Resembles that of GITR (Figure 2A)
 
OX40 (CD134), GITR**, CD30#, CD69, CD103, CD122, CD150
 

Staining pattern versus CD25*for cells gated for expression of CD4

Antibodies
All cells negative or very low   CD8, CD21, CD23, CD40L, CD54, CD70§, CD74, CD84, CXCR5, CD148 
Large negative population, including all CD25+ cells   CD56, CD95L, CD244§, CXCR4 
All cells positive   CD3 
Most cells positive, including all CD25+ cells   CD5, CD11a, CD28#, CD57 
  CD27, CD38, CD44, CD45RA, CD45RO, CD58, CD62L, CD71, CD95,  
Profiles shown in Figure 2   CTLA-4 (CD152), HLA-DR, CCR7 
Resembles that of GITR (Figure 2A)
 
OX40 (CD134), GITR**, CD30#, CD69, CD103, CD122, CD150
 
*

Obtained from BD Biosciences, San Jose, CA

Obtained from PharMingen

Obtained from Ancell, Bayport, MN

§

Obtained from Immunotech, Marseille, France

Obtained from DNAX Research Institute, Palo Alto, CA

Obtained from Caltag, Burlingame, CA

#

Obtained from Miltenyi Biotec, Gladbach, Germany

**

Obtained from R&D Systems, Minneapolis, MN

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