X-ray data collection and refinement statistics
| . | Flt3D1-D4:FL . | Flt3D1-D5:FL . |
|---|---|---|
| Data collection | ||
| Source, wavelength (Å) | ESRF ID23-1, 0.9762 | ESRF ID23-1, 1.0762 |
| Detector | ADSC-Q315R | ADSC-Q315R |
| Resolution (Å) | 40.00-4.30 (4.45-4.30) | 35.00-7.80 (8.00-7.80) |
| Space group | P21 | P21 |
| Unit cell parameters | ||
| a | a = 103.89 | a = 124.75 |
| b | b = 146.26 | b = 153.55 |
| c | c = 105.95 | c = 133.87 |
| α = γ = 90°, β = 109.7° | α = γ = 90°, β = 94.6° | |
| Wilson B (Å2) | 135 | 401 |
| Unique reflections | 20 184 (1942) | 5656 (382) |
| Redundancy | 3.8 (3.8) | 3.4 (3.2) |
| Completeness (%) | 98.8 (98.9) | 96.6 (92.9) |
| Rmeas (%)* | 10.8 (75.9) | 12.8 (80.7) |
| Average I/σ(I) | 12.04 (2.08) | 9.1 (1.9) |
| Refinement | ||
| Resolution (Å) | 40.00-4.30 (4.53-4.30) | 35.00-7.80 (8.72-7.80) |
| Reflections working set/test set | 19 172/1010 (2763/141) | 5090/565 (1437/159) |
| Rwork, Rfree | 0.260/0.281 (0.268/0.268) | 0.337/0.346 (0.334/0.313) |
| Root-mean-square deviations | ||
| Bonds (Å) | 0.010 | n.a. |
| Angles (°) | 1.12 | n.a. |
| Average ADP (Å2) | 169 | 364 |
| Ramachandran analysis (%) | ||
| Favorable | 89.8 | n.a. |
| Outliers | 2.0 | n.a. |
| PDB access code | 3QS7 | 3QS9 |
| . | Flt3D1-D4:FL . | Flt3D1-D5:FL . |
|---|---|---|
| Data collection | ||
| Source, wavelength (Å) | ESRF ID23-1, 0.9762 | ESRF ID23-1, 1.0762 |
| Detector | ADSC-Q315R | ADSC-Q315R |
| Resolution (Å) | 40.00-4.30 (4.45-4.30) | 35.00-7.80 (8.00-7.80) |
| Space group | P21 | P21 |
| Unit cell parameters | ||
| a | a = 103.89 | a = 124.75 |
| b | b = 146.26 | b = 153.55 |
| c | c = 105.95 | c = 133.87 |
| α = γ = 90°, β = 109.7° | α = γ = 90°, β = 94.6° | |
| Wilson B (Å2) | 135 | 401 |
| Unique reflections | 20 184 (1942) | 5656 (382) |
| Redundancy | 3.8 (3.8) | 3.4 (3.2) |
| Completeness (%) | 98.8 (98.9) | 96.6 (92.9) |
| Rmeas (%)* | 10.8 (75.9) | 12.8 (80.7) |
| Average I/σ(I) | 12.04 (2.08) | 9.1 (1.9) |
| Refinement | ||
| Resolution (Å) | 40.00-4.30 (4.53-4.30) | 35.00-7.80 (8.72-7.80) |
| Reflections working set/test set | 19 172/1010 (2763/141) | 5090/565 (1437/159) |
| Rwork, Rfree | 0.260/0.281 (0.268/0.268) | 0.337/0.346 (0.334/0.313) |
| Root-mean-square deviations | ||
| Bonds (Å) | 0.010 | n.a. |
| Angles (°) | 1.12 | n.a. |
| Average ADP (Å2) | 169 | 364 |
| Ramachandran analysis (%) | ||
| Favorable | 89.8 | n.a. |
| Outliers | 2.0 | n.a. |
| PDB access code | 3QS7 | 3QS9 |
The values in parentheses refer to the highest resolution shell.
ADP indicates Atomic Displacement Parameters; and n.a., not applicable.
Rmeas = Σh√nh/(nh − 1) ΣhΣi| I(h,i) −<I(h)>| /ΣhΣiI(h,i), where nh is the multiplicity, I(h,i) is the intensity of the ith measurement of reflection h, and <I(h)> is the average value over multiple measurements.