RV-mediated tk transduction and induction of human mH antigen-specific CTLs
| Condition* . | Transduction,† (%) . | Yields,‡ ×106 . | CD4/CD8,§ n . | HLA-A2HA-1 tet+,¶ % . | HLA-A2H-Y tet+,¶ % . |
|---|---|---|---|---|---|
| PBL | — | 10 | 2.1 ± 0.3 | 2.4 (2.1-3.5) | 2.7 (1.4-3.7) |
| OKT3 | 18.4 ± 4.6 | 23 ± 6.3‖ | 1.1 ± 0.2‖ | 0.7 (0.4-1.2) | 0.5 (0.2-0.9) |
| B + IL-2 | 41.1 ± 8.1‖ | 65 ± 11.3‖ | 1.8 ± 0.2 | 1.0 (0.4-1.4) | 0.8 (0.3-1.2) |
| B + IL-7 | 36.7 ± 7.5‖ | 47.3 ± 6.4‖ | 2.2 ± 0.2 | 0.3 (0.1-0.5) | 0.7 (0.5-0.9) |
| B + IL-7/IL-15 | 40.1 ± 9.6‖ | 70.1 ± 11.3‖ | 1.8 ± 0.2 | 3.1 (2.8-4.1) | 2.4 (2.1-4.7) |
| Condition* . | Transduction,† (%) . | Yields,‡ ×106 . | CD4/CD8,§ n . | HLA-A2HA-1 tet+,¶ % . | HLA-A2H-Y tet+,¶ % . |
|---|---|---|---|---|---|
| PBL | — | 10 | 2.1 ± 0.3 | 2.4 (2.1-3.5) | 2.7 (1.4-3.7) |
| OKT3 | 18.4 ± 4.6 | 23 ± 6.3‖ | 1.1 ± 0.2‖ | 0.7 (0.4-1.2) | 0.5 (0.2-0.9) |
| B + IL-2 | 41.1 ± 8.1‖ | 65 ± 11.3‖ | 1.8 ± 0.2 | 1.0 (0.4-1.4) | 0.8 (0.3-1.2) |
| B + IL-7 | 36.7 ± 7.5‖ | 47.3 ± 6.4‖ | 2.2 ± 0.2 | 0.3 (0.1-0.5) | 0.7 (0.5-0.9) |
| B + IL-7/IL-15 | 40.1 ± 9.6‖ | 70.1 ± 11.3‖ | 1.8 ± 0.2 | 3.1 (2.8-4.1) | 2.4 (2.1-4.7) |
Conditions for transduction: OKT3, anti-CD3 mAb, and IL-2; B + IL-2, CD3/CD28-beads and IL-2; B + IL-7, CD3/CD28-beads and IL-7; B + IL-7/IL-15, CD3/CD28-beads, IL-7, and IL-15
Transduction efficiency
NGFR+ T-cell yields after transduction
CD4/CD8 ratio on NGFR+ cells
HLA-A2HA-1 or HLA-A2H-Y tetramer binding on NGFR+/CD8+ T cells
indicates P < .05