Table 2 Cytotoxicity and expansion of... | American Society of Hematology

Table 2

Cytotoxicity and expansion of human tk⁺ mH antigen-specific CTLs after IL-7Rα sequential sorting

CTLs*	IL-7Rα⁺,† %	Sorting			IL-7Rα⁺,† %	Re-sorting
CTLs*	IL-7Rα⁺,† %	IL-7Rα,‡ %	Cytotoxicity,§ %	Expansion,¶ fold	IL-7Rα⁺,† %	IL-7Rα,‡ %	Cytotoxicity,§ %	Expansion,¶ fold
HA1-tk #1	32.9	92.4^pos	12.7	17.6	12.6	95.3^pos	21.2	10.4
HA1-tk #1	32.9	< 1^neg	65.7	4.1	12.6	< 1^neg	78.5	4.7
HA1-tk #2	54.6	86.7^pos	16.8	24.2	10.9	91.7^pos	11.5	14.5
HA1-tk #2	54.6	2.3^neg	54.2	4.6	10.9	1.8^neg	84.5	—
HY-tk #1	21.7	81.8^pos	21.2	35.1	15.4	89.6^pos	22.6	12.3
HY-tk #1	21.7	5.6^neg	74.0	8.7	15.4	1.5^neg	81.2	3.5

CTLs*	IL-7Rα⁺,† %	Sorting			IL-7Rα⁺,† %	Re-sorting
CTLs*	IL-7Rα⁺,† %	IL-7Rα,‡ %	Cytotoxicity,§ %	Expansion,¶ fold	IL-7Rα⁺,† %	IL-7Rα,‡ %	Cytotoxicity,§ %	Expansion,¶ fold
HA1-tk #1	32.9	92.4^pos	12.7	17.6	12.6	95.3^pos	21.2	10.4
HA1-tk #1	32.9	< 1^neg	65.7	4.1	12.6	< 1^neg	78.5	4.7
HA1-tk #2	54.6	86.7^pos	16.8	24.2	10.9	91.7^pos	11.5	14.5
HA1-tk #2	54.6	2.3^neg	54.2	4.6	10.9	1.8^neg	84.5	—
HY-tk #1	21.7	81.8^pos	21.2	35.1	15.4	89.6^pos	22.6	12.3
HY-tk #1	21.7	5.6^neg	74.0	8.7	15.4	1.5^neg	81.2	3.5

Thymidine kinase–positive CTLs used for sorting: HA1-tk #1, specific for HA-1 and induced from an HLA-A2^pos/HA-1^neg donor; HA1-tk #2, specific for HA-1 and induced from a second HLA-A2^pos/HA-1^neg donor; HY-tk #1, specific for H-Y and induced from an HLA-A2^pos/H-Y^neg (female) donor

†

Thymidine kinase–positive mH antigen-specific CTLs expressing IL-7Rα

‡

Thymidine kinase–positive mH antigen-specific CTLs expressing IL-7Rα in the positive (pos) and in the negative (neg) fraction after IL-7Rα sorting

Cytotoxicity of IL-7Rα⁺ and IL-7Rα⁻tk⁺ mH antigen-specific CTLs against an LCL expressing the relevant mH antigen at a 20:1 ratio

Expansion of IL-7Rα⁺ and IL-7Rα⁻tk⁺ mH antigen-specific CTLs

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