Table 1 Primer sequences used for... | American Society of Hematology

Table 1

Primer sequences used for quantitative PCR reactions

Gene	Primer sequence
Human KLF2	Forward: 5′-TGCGGCAAGACCTACACCAAGAGT3-′
	Reverse: 5′-AGCCGCAGCCGTCCCAGTT3-′
Human KLF4	Forward: 5′-ACCAGGCAC TACCGTAAACACA3-′
	Reverse: 5′-GGTCCGACCTGGAAAATGCT3-′
Human GAPDH	Forward: 5′-GCCATCAATGACCCCTTCATT3-′
	Reverse: 5′-TCTCGCTCCTGG AAGATGG3-′
Human E-selectin	Forward: 5′-TGCGATGCTGCCTACTTGTG3-′
	Reverse: 5′-AGAGAGTGCCACTACCAAGGGA3-′
NF-κB promoter in human E-selectin (for ChIP)	Forward: 5′-AGGCATGGACAAAGGTGAAG3-′
NF-κB promoter in human E-selectin (for ChIP)	Reverse: 5′-CCAATGGCATCCAAAAACTT3-′

Gene	Primer sequence
Human KLF2	Forward: 5′-TGCGGCAAGACCTACACCAAGAGT3-′
	Reverse: 5′-AGCCGCAGCCGTCCCAGTT3-′
Human KLF4	Forward: 5′-ACCAGGCAC TACCGTAAACACA3-′
	Reverse: 5′-GGTCCGACCTGGAAAATGCT3-′
Human GAPDH	Forward: 5′-GCCATCAATGACCCCTTCATT3-′
	Reverse: 5′-TCTCGCTCCTGG AAGATGG3-′
Human E-selectin	Forward: 5′-TGCGATGCTGCCTACTTGTG3-′
	Reverse: 5′-AGAGAGTGCCACTACCAAGGGA3-′
NF-κB promoter in human E-selectin (for ChIP)	Forward: 5′-AGGCATGGACAAAGGTGAAG3-′
NF-κB promoter in human E-selectin (for ChIP)	Reverse: 5′-CCAATGGCATCCAAAAACTT3-′