Table 1 Binding affinity of WT, E325K,... | American Society of Hematology

Table 1

Binding affinity of WT, E325K, and K332Q EKLF to a range of promoter EKLF recognition sequences

Promoter	EKLF recognition sequence	K_{d, nM}			Fold difference between WT and E325K	Fold difference between WT and K332Q
Promoter	EKLF recognition sequence	WT	E325K	K332Q	Fold difference between WT and E325K	Fold difference between WT and K332Q
HBB	CCA CAC CCT	122*	1286	156	10.5	1.3
LU	CCC CAC CCC	332	1564	938	4.7	2.8
CD44	CCG CGC CCA	1225	—	—	—	—
ICAM4	CTC CAC CCT	187	788	426	4.2	2.3
AQP1	CCC CAC CCA	245	870	550	3.6	2.2
BCL11A	TCC CAC CCC	461	979	1350	2.1	2.9
p21	CCC CGC CCG	537	—	782	—	1.5

Promoter	EKLF recognition sequence	K_{d, nM}			Fold difference between WT and E325K	Fold difference between WT and K332Q
Promoter	EKLF recognition sequence	WT	E325K	K332Q	Fold difference between WT and E325K	Fold difference between WT and K332Q
HBB	CCA CAC CCT	122*	1286	156	10.5	1.3
LU	CCC CAC CCC	332	1564	938	4.7	2.8
CD44	CCG CGC CCA	1225	—	—	—	—
ICAM4	CTC CAC CCT	187	788	426	4.2	2.3
AQP1	CCC CAC CCA	245	870	550	3.6	2.2
BCL11A	TCC CAC CCC	461	979	1350	2.1	2.9
p21	CCC CGC CCG	537	—	782	—	1.5

— indicates that the binding is too low to calculate the K_d value.

Nine repeats from Figures 2 and 3 combined.