Incorporation of 3H-thymidin in spleen cells restimulated for 3 days with human FVIII in vitro
| Mouse strain . | Treatment . | FVIII in culture, μg/mL . | 3H incorporation, cpm . | Stimulation index . | |
|---|---|---|---|---|---|
| mean (n = 3) . | SD . | ||||
| E17F8ko | FVIII | 0 | 5.020 | 460 | 1 |
| 1 | 8.586 | 2.075 | 1.70 | ||
| 10 | 9.252 | 2.333 | 1.84 | ||
| E17F8ko | mFVIII1 | 0 | 4.758 | 401 | 1 |
| 1 | 6.272 | 2.210 | 1.32 | ||
| 10 | 5.518 | 999 | 1.16 | ||
| E17F8ko | mFVIII2 | 0 | 5.666 | 939 | 1 |
| 1 | 34.257 | 2.788 | 6.05* | ||
| 10 | 31.402 | 2.170 | 5.54* | ||
| huF8 | FVIII | 0 | 3.647 | 671 | 1 |
| 1 | 4.002 | 452 | 1.10 | ||
| 10 | 4.297 | 1.721 | 1.18 | ||
| huF8 | mFVIII1 | 0 | 5.041 | 505 | 1 |
| 1 | 6.215 | 502 | 1.23 | ||
| 10 | 4.714 | 530 | 0.94 | ||
| huF8 | mFVIII2 | 0 | 4.757 | 268 | 1 |
| 1 | 4.723 | 466 | 0.99 | ||
| 10 | 5.767 | 1.167 | 1.21 | ||
| Mouse strain . | Treatment . | FVIII in culture, μg/mL . | 3H incorporation, cpm . | Stimulation index . | |
|---|---|---|---|---|---|
| mean (n = 3) . | SD . | ||||
| E17F8ko | FVIII | 0 | 5.020 | 460 | 1 |
| 1 | 8.586 | 2.075 | 1.70 | ||
| 10 | 9.252 | 2.333 | 1.84 | ||
| E17F8ko | mFVIII1 | 0 | 4.758 | 401 | 1 |
| 1 | 6.272 | 2.210 | 1.32 | ||
| 10 | 5.518 | 999 | 1.16 | ||
| E17F8ko | mFVIII2 | 0 | 5.666 | 939 | 1 |
| 1 | 34.257 | 2.788 | 6.05* | ||
| 10 | 31.402 | 2.170 | 5.54* | ||
| huF8 | FVIII | 0 | 3.647 | 671 | 1 |
| 1 | 4.002 | 452 | 1.10 | ||
| 10 | 4.297 | 1.721 | 1.18 | ||
| huF8 | mFVIII1 | 0 | 5.041 | 505 | 1 |
| 1 | 6.215 | 502 | 1.23 | ||
| 10 | 4.714 | 530 | 0.94 | ||
| huF8 | mFVIII2 | 0 | 4.757 | 268 | 1 |
| 1 | 4.723 | 466 | 0.99 | ||
| 10 | 5.767 | 1.167 | 1.21 | ||
Spleen cells were obtained from conventional hemophilic E17 F8-knockout mice or human F8-transgenic mice at 1 week after the last dose of 9 weekly treatments with either human FVIII or 1 of 2 PEGylated FVIII preparations (mFVIII1 and mFVIII2).
P < .01.