BCR variable-domain analysis of tetramer-positive memory B cells isolated from donor 1
| ID . | Heavy chain . | Kappa light chain . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| V-D-J junction . | V . | D . | J . | V-Mut nt/aa* . | CDRs† . | V-J junction . | V . | J . | V-Mut nt/aa* . | CDRs† . | |
| M1‡ | CARVRAVVRMQHYYYGMDLW | V1-2 | D3-10 | J6 | 18/13 | 8.8.18 | CMQALQTPPTF | V2-28 or V2D-28 | J1 | 5/3 | 11.3.9 |
| M2‡ | CARVRAVVRTQHYYYGMDLW | V1-2 | D3-10 | J6 | 18/13 | 8.8.18 | CMQALQTPPTF | V2-28 or V2D-28 | J1 | 5/3 | 11.3.9 |
| M3 | CAGGGYTNPLIIG | V3-23 | D4-4 | J4 | 42/22 | 8.8.11 | CQQYANSPRTF | V3-20 | J1 | 14/6 | 7.3.9 |
| M4 | CARDGAPYTRKDRFDPW | V3-33 | D1-7 | J5 | 12/9 | 8.8.15 | CQQYNTYSPWTF | V1-5 | J1 | 5/2 | 6.3.10 |
| M5 | CARSGGSASGSFYPGALYYNGMDVW | V4-4 | D3-10 | J6 | 25/11 | 8.7.23 | CQQSYSATWTF | V1-39 or V1D-39 | J1 | 23/11 | 6.3.9 |
| M6 | CATEPGLHCGPDCPDAFEIW | V4-31 | D2-21 | J3 | 20/13 | 10.7.18 | CQHHHTF | V1-9 | J2 | 17/10 | 6.3.5 |
| M7 | CARGFMVTLGGVIEHNGLHIW | V4-31 | D3-16 | J3 | 21/13 | 10.7.19 | CQQRSNWPPVPTF | V3-11 | J3 | 7/6 | 6.3.11 |
| M8 | CGRLWGRSYSYYSGSYYENFDYW | V5-51 | D3-10 | J4 | 21/14 | 8.8.21 | CHQYGSLPPTF | V3-20 | J1 | 35/20 | 7.3.9 |
| M9 | CARPSNYDFWSGYYETGYAFDMW | V5-51 | D3-3 | J3 | 8/4 | 8.8.21 | CQQYGSLPRTF | V3-20 | J1 | 9/3 | 7.3.9 |
| M10 | CQQSHNTPLTF | V1-39 or V1D-39 | J4 | 17/11 | 6.3.9 | ||||||
| M11 | CQQSFSTLYTF | V1-39 or V1D-39 | J2 | 6/3 | 6.3.9 | ||||||
| ID . | Heavy chain . | Kappa light chain . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| V-D-J junction . | V . | D . | J . | V-Mut nt/aa* . | CDRs† . | V-J junction . | V . | J . | V-Mut nt/aa* . | CDRs† . | |
| M1‡ | CARVRAVVRMQHYYYGMDLW | V1-2 | D3-10 | J6 | 18/13 | 8.8.18 | CMQALQTPPTF | V2-28 or V2D-28 | J1 | 5/3 | 11.3.9 |
| M2‡ | CARVRAVVRTQHYYYGMDLW | V1-2 | D3-10 | J6 | 18/13 | 8.8.18 | CMQALQTPPTF | V2-28 or V2D-28 | J1 | 5/3 | 11.3.9 |
| M3 | CAGGGYTNPLIIG | V3-23 | D4-4 | J4 | 42/22 | 8.8.11 | CQQYANSPRTF | V3-20 | J1 | 14/6 | 7.3.9 |
| M4 | CARDGAPYTRKDRFDPW | V3-33 | D1-7 | J5 | 12/9 | 8.8.15 | CQQYNTYSPWTF | V1-5 | J1 | 5/2 | 6.3.10 |
| M5 | CARSGGSASGSFYPGALYYNGMDVW | V4-4 | D3-10 | J6 | 25/11 | 8.7.23 | CQQSYSATWTF | V1-39 or V1D-39 | J1 | 23/11 | 6.3.9 |
| M6 | CATEPGLHCGPDCPDAFEIW | V4-31 | D2-21 | J3 | 20/13 | 10.7.18 | CQHHHTF | V1-9 | J2 | 17/10 | 6.3.5 |
| M7 | CARGFMVTLGGVIEHNGLHIW | V4-31 | D3-16 | J3 | 21/13 | 10.7.19 | CQQRSNWPPVPTF | V3-11 | J3 | 7/6 | 6.3.11 |
| M8 | CGRLWGRSYSYYSGSYYENFDYW | V5-51 | D3-10 | J4 | 21/14 | 8.8.21 | CHQYGSLPPTF | V3-20 | J1 | 35/20 | 7.3.9 |
| M9 | CARPSNYDFWSGYYETGYAFDMW | V5-51 | D3-3 | J3 | 8/4 | 8.8.21 | CQQYGSLPRTF | V3-20 | J1 | 9/3 | 7.3.9 |
| M10 | CQQSHNTPLTF | V1-39 or V1D-39 | J4 | 17/11 | 6.3.9 | ||||||
| M11 | CQQSFSTLYTF | V1-39 or V1D-39 | J2 | 6/3 | 6.3.9 | ||||||
Bolded sequence pair represents clonally related B cells.
CDR indicates Ca2+-dependent regulator.
Number of mutations detected in V-gene segment (nucleotides/amino acids).
CDR1, 2, and 3 lengths in amino acids.
Identical VH-D-JH and Vκ-Jκ gene rearrangements in both heavy and light chains, respectively.