Intracellular cytokine mAbs
| mAB specificity (clone) . | Stain . | Ig subclass . | Conjugate . | Source . |
|---|---|---|---|---|
| CD19 (HIb19)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | eBioscience |
| CD14 (TUK4)* | Surface (dump) | Mouse IgG2a | Q800 | Invitrogen |
| CD56 (MEM188) | Surface | Mouse IgG2a | PE-Cy5.5 | eBioscience |
| CD4 (S3.5) | Surface | Mouse IgG1 | PE-Texas Red | Invitrogen |
| CD8 (3B5) | Surface | Mouse IgG2a | Q705 | Invitrogen |
| CD45RA (MEM-56) | Surface | Mouse IgG2b | Q655 | Invitrogen |
| IL-4 (MP4–25D2) | Intracellular | Rat IgG1 | PE | BD PharMingen |
| IFN-γ (B27) | Intracellular | Mouse IgG1 | PE-Cy7 | BD PharMingen |
| CD3 (UCHT1) | Intracellular | Mouse IgG2a | Q605 | Invitrogen |
| TNF-α (Mab11) | Intracellular | Mouse IgG1 | PerCP-Cy5.5 | Biolegend |
| IL-17a (BL168) | Intracellular | Mouse IgG1 | Pacific Blue | Biolegend |
| IL-10 (JES3–19F1) | Intracellular | Rat IgG2a | APC | Biolegend |
| IL-2 (MQ1–17H12) | Intracellular | Rat IgG2a | Alexa700 | Biolegend |
| CD69 (FN50) | Intracellular | Mouse IgG1 | APC-Cy7 | Biolegend |
| MIP-1β (24 006) | Intracellular | Mouse IgG2b | FITC | R&D Systems |
| Live/dead aqua fluorescent reactive dye* | Vital dye | 405 nm | Invitrogen |
| mAB specificity (clone) . | Stain . | Ig subclass . | Conjugate . | Source . |
|---|---|---|---|---|
| CD19 (HIb19)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | eBioscience |
| CD14 (TUK4)* | Surface (dump) | Mouse IgG2a | Q800 | Invitrogen |
| CD56 (MEM188) | Surface | Mouse IgG2a | PE-Cy5.5 | eBioscience |
| CD4 (S3.5) | Surface | Mouse IgG1 | PE-Texas Red | Invitrogen |
| CD8 (3B5) | Surface | Mouse IgG2a | Q705 | Invitrogen |
| CD45RA (MEM-56) | Surface | Mouse IgG2b | Q655 | Invitrogen |
| IL-4 (MP4–25D2) | Intracellular | Rat IgG1 | PE | BD PharMingen |
| IFN-γ (B27) | Intracellular | Mouse IgG1 | PE-Cy7 | BD PharMingen |
| CD3 (UCHT1) | Intracellular | Mouse IgG2a | Q605 | Invitrogen |
| TNF-α (Mab11) | Intracellular | Mouse IgG1 | PerCP-Cy5.5 | Biolegend |
| IL-17a (BL168) | Intracellular | Mouse IgG1 | Pacific Blue | Biolegend |
| IL-10 (JES3–19F1) | Intracellular | Rat IgG2a | APC | Biolegend |
| IL-2 (MQ1–17H12) | Intracellular | Rat IgG2a | Alexa700 | Biolegend |
| CD69 (FN50) | Intracellular | Mouse IgG1 | APC-Cy7 | Biolegend |
| MIP-1β (24 006) | Intracellular | Mouse IgG2b | FITC | R&D Systems |
| Live/dead aqua fluorescent reactive dye* | Vital dye | 405 nm | Invitrogen |
Both antibodies run in same channel as vital dye.