Checkerboard analysis for inhibition of migration by anti-Plg-RKT 7H1 treatment
. | Well . | Cells × 104 . | |
---|---|---|---|
Isotype control . | 7H1 . | ||
Buffer | 0.838 ± 0.0211 | 0.7128 ± 0.0754 | |
MCP-1 | Lower | 2.258 ± 0.0552 | 1.4860 ± 0.1070 |
MCP-1 | Upper & lower | 2.159 ± 0.0614 | 0.9857 ± 0.0535 |
. | Well . | Cells × 104 . | |
---|---|---|---|
Isotype control . | 7H1 . | ||
Buffer | 0.838 ± 0.0211 | 0.7128 ± 0.0754 | |
MCP-1 | Lower | 2.258 ± 0.0552 | 1.4860 ± 0.1070 |
MCP-1 | Upper & lower | 2.159 ± 0.0614 | 0.9857 ± 0.0535 |
U937 cells were placed in upper chambers of transwells and migration in the presence of plasminogen (200 nM) was quantified in response to MCP-1 (in either the lower or upper and lower chambers) or buffer, as described in “Invasion and chemotactic migration.” Means ± SEM are shown.