Table 1

Checkerboard analysis for inhibition of migration by anti-Plg-RKT 7H1 treatment

WellCells × 104
Isotype control7H1
Buffer  0.838 ± 0.0211 0.7128 ± 0.0754 
    MCP-1 Lower 2.258 ± 0.0552 1.4860 ± 0.1070 
    MCP-1 Upper & lower 2.159 ± 0.0614 0.9857 ± 0.0535 
WellCells × 104
Isotype control7H1
Buffer  0.838 ± 0.0211 0.7128 ± 0.0754 
    MCP-1 Lower 2.258 ± 0.0552 1.4860 ± 0.1070 
    MCP-1 Upper & lower 2.159 ± 0.0614 0.9857 ± 0.0535 

U937 cells were placed in upper chambers of transwells and migration in the presence of plasminogen (200 nM) was quantified in response to MCP-1 (in either the lower or upper and lower chambers) or buffer, as described in “Invasion and chemotactic migration.” Means ± SEM are shown.

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