Decitabine-induced change in DNA methylation
| Cell population locus . | BMCD34P . | BMCD34N . | BMMC Change in methylation (%), significance . | PBMC . | GRANS . |
|---|---|---|---|---|---|
| HIST1H2AA | −32, P = .001 | −16, P = .075 | −30, P = .005 | −25, P = .020 | 8, P = .67 |
| LINE1 | −19, P = .068 | −24, P = .012 | −46, P = .012 | −28, P = .001 | −14, P = .11 |
| Cell population locus . | BMCD34P . | BMCD34N . | BMMC Change in methylation (%), significance . | PBMC . | GRANS . |
|---|---|---|---|---|---|
| HIST1H2AA | −32, P = .001 | −16, P = .075 | −30, P = .005 | −25, P = .020 | 8, P = .67 |
| LINE1 | −19, P = .068 | −24, P = .012 | −46, P = .012 | −28, P = .001 | −14, P = .11 |
For each specimen type, DNA methylation at the HIST1H2AA locus and LINE1 repeat class was compared before (screening) and after (post-priming) decitabine priming. The negative -fold change reflects the reduction in DNA methylation induced by decitabine. Statistical significance (P) of the -fold change was calculated using a one-sided Student t test for the paired specimens.