Changes in the EpCAM+ MHC II+ and EpCAM+ MHC IIhi TEC with age
| Mouse strain . | EpCAM+ MHC II+ TEC cell number . | EpCAM+ MHC IIhi TEC . | ||||
|---|---|---|---|---|---|---|
| Total cell number . | % Ki-67 positive . | |||||
| 3 mo . | 18-20 mo . | 3 mo . | 18-20 mo . | 3 mo . | 18-20 mo . | |
| WT | 27 481 (11 622) | 7400 (1871)† | 4996 (902) | 554 (175)¶ | 53.1 (21) | 15.1 (5.4)†† |
| Foxn1Tg | 68 149 (18 842)* | 15 189 (4159)‡,§ | 25 600 (4860)‖ | 1044 (297)#,** | 56.7 (7.3) | 29.4 (12.5)‡‡,§§ |
| Mouse strain . | EpCAM+ MHC II+ TEC cell number . | EpCAM+ MHC IIhi TEC . | ||||
|---|---|---|---|---|---|---|
| Total cell number . | % Ki-67 positive . | |||||
| 3 mo . | 18-20 mo . | 3 mo . | 18-20 mo . | 3 mo . | 18-20 mo . | |
| WT | 27 481 (11 622) | 7400 (1871)† | 4996 (902) | 554 (175)¶ | 53.1 (21) | 15.1 (5.4)†† |
| Foxn1Tg | 68 149 (18 842)* | 15 189 (4159)‡,§ | 25 600 (4860)‖ | 1044 (297)#,** | 56.7 (7.3) | 29.4 (12.5)‡‡,§§ |
The numbers of EpCAM+ MHC II+ and MHC IIhi TEC cells were calculated based on the total cell number in the CD45− compartment and the percentages of EpCAM+ cells within the MHC II+ and MHC IIhi subsets as determined by flow cytometry. Data represent mean (SD) values calculated from 5 young (3-month-old) WT, Foxn1Tg, old (18–22 months) Foxn1 Tg mice, and 4 old WT mice. Statistical significance was determined by t test: *P = .003, vs young WT; †P = .012, vs young WT; ‡P = .016, vs young Foxn1 Tg; §P = .023, vs old WT; ‖P < .001, vs young WT; ¶P < .001, vs young WT; #P = .016, vs young Foxn1 Tg; **P = .040, vs old WT; ††P < .001, vs young WT; ‡‡P = .002, vs young Foxn1 Tg; and §§P = .017, vs old WT.