Serum and hematologic parameters of Fpn1flox/flox and Fpn1LysM/LysM mice
| . | 2-month-old mice . | 10-month-old mice . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Fpn1flox/flox . | n . | Fpn1LysM/LysM . | n . | P . | Fpn1flox/flox . | n . | Fpn1LysM/LysM . | n . | P . | |
| Serum parameters | ||||||||||
| Serum iron, μg/dL | 238 ± 37 | 12 | 186 ± 34 | 12 | .0016 | 128 ± 4 | 6 | 67 ± 33 | 6 | .0108 |
| UIBC, μg/dL | 192 ± 25 | 12 | 225 ± 54 | 12 | .0680 | 230 ± 35 | 6 | 332 ± 32 | 6 | .0050 |
| TIBC, μg/dL | 430 ± 44 | 12 | 411 ± 49 | 12 | .3225 | 357 ± 36 | 6 | 399 ± 38 | 6 | .1580 |
| TS, % | 55.1 ± 5.1 | 12 | 45.7 ± 9.5 | 12 | .0063 | 36.0 ± 3.6 | 6 | 16.5 ± 7.2 | 6 | .0028 |
| Hematology | ||||||||||
| WBCs, × 109/L | 2.00 ± 0.70 | 10 | 1.99 ± 1.13 | 10 | .9848 | 2.83 ± 1.03 | 6 | 3.15 ± 1.23 | 6 | .6989 |
| RBCs, × 1012/L | 10.3 ± 1.1 | 10 | 8.9 ± 1.2 | 10 | .0380 | 7.8 ± 0.5 | 6 | 7.9 ± 0.4 | 6 | .6753 |
| Hb, g/L | 171 ± 15 | 10 | 143 ± 17 | 10 | .0060 | 126 ± 9 | 6 | 114 ± 9 | 6 | .1008 |
| Hct | 0.54 ± 0.06 | 10 | 0.45 ± 0.06 | 10 | .0120 | 0.41 ± 0.02 | 6 | 0.37 ± 0.1 | 6 | .0798 |
| MCV, fL | 52.9 ± 0.6 | 10 | 51.2 ± 0.9 | 10 | .0010 | 52.3 ± 0.3 | 6 | 46.7 ± 2.4 | 6 | .0038 |
| MCH, pg | 16.7 ± 0.5 | 10 | 16.2 ± 0.54 | 10 | .1110 | 16.2 ± 0.33 | 6 | 14.4 ± 0.98 | 6 | .0112 |
| MCHC, g/L | 315 ± 8.0 | 10 | 316 ± 9.0 | 10 | .7910 | 310 ± 7.0 | 6 | 308 ± 6.0 | 6 | .6635 |
| RDW, % | 14.5 ± 2.0 | 10 | 13.5 ± 1.0 | 10 | .167 | 13.8 ± 0.8 | 6 | 17.5 ± 3.9 | 6 | .045 57 |
| . | 2-month-old mice . | 10-month-old mice . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Fpn1flox/flox . | n . | Fpn1LysM/LysM . | n . | P . | Fpn1flox/flox . | n . | Fpn1LysM/LysM . | n . | P . | |
| Serum parameters | ||||||||||
| Serum iron, μg/dL | 238 ± 37 | 12 | 186 ± 34 | 12 | .0016 | 128 ± 4 | 6 | 67 ± 33 | 6 | .0108 |
| UIBC, μg/dL | 192 ± 25 | 12 | 225 ± 54 | 12 | .0680 | 230 ± 35 | 6 | 332 ± 32 | 6 | .0050 |
| TIBC, μg/dL | 430 ± 44 | 12 | 411 ± 49 | 12 | .3225 | 357 ± 36 | 6 | 399 ± 38 | 6 | .1580 |
| TS, % | 55.1 ± 5.1 | 12 | 45.7 ± 9.5 | 12 | .0063 | 36.0 ± 3.6 | 6 | 16.5 ± 7.2 | 6 | .0028 |
| Hematology | ||||||||||
| WBCs, × 109/L | 2.00 ± 0.70 | 10 | 1.99 ± 1.13 | 10 | .9848 | 2.83 ± 1.03 | 6 | 3.15 ± 1.23 | 6 | .6989 |
| RBCs, × 1012/L | 10.3 ± 1.1 | 10 | 8.9 ± 1.2 | 10 | .0380 | 7.8 ± 0.5 | 6 | 7.9 ± 0.4 | 6 | .6753 |
| Hb, g/L | 171 ± 15 | 10 | 143 ± 17 | 10 | .0060 | 126 ± 9 | 6 | 114 ± 9 | 6 | .1008 |
| Hct | 0.54 ± 0.06 | 10 | 0.45 ± 0.06 | 10 | .0120 | 0.41 ± 0.02 | 6 | 0.37 ± 0.1 | 6 | .0798 |
| MCV, fL | 52.9 ± 0.6 | 10 | 51.2 ± 0.9 | 10 | .0010 | 52.3 ± 0.3 | 6 | 46.7 ± 2.4 | 6 | .0038 |
| MCH, pg | 16.7 ± 0.5 | 10 | 16.2 ± 0.54 | 10 | .1110 | 16.2 ± 0.33 | 6 | 14.4 ± 0.98 | 6 | .0112 |
| MCHC, g/L | 315 ± 8.0 | 10 | 316 ± 9.0 | 10 | .7910 | 310 ± 7.0 | 6 | 308 ± 6.0 | 6 | .6635 |
| RDW, % | 14.5 ± 2.0 | 10 | 13.5 ± 1.0 | 10 | .167 | 13.8 ± 0.8 | 6 | 17.5 ± 3.9 | 6 | .045 57 |
Data are mean ± SEM. The sample size (n) is indicated. P represents the value of a Student t test (unpaired, 2-tailed). Serum iron status and hematologic parameters in 2-month-old (Fpn1flox/flox, 6 male, 6 female; Fpn1LysM/LysM, 6 male, 6 female) and 10-month-old (Fpn1flox/flox, 3 male, 3 female; Fpn1LysM/LysM, 3 male, 3 female) mice indicate a mild iron deficiency anemia in Fpn1 knockout animals.
TIBC indicates total iron-binding capacity; TS, transferrin saturation; WBCs, white blood cells; RBCs, red blood cells; Hb, hemoglobin; Hct, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; and RDW, red blood cell distribution width.