Table 1 Immunophenotype of the cells... | American Society of Hematology

Table 1

Immunophenotype of the cells obtained after 6 week LTC-IC-HE of adult human purified CD34⁻Lin⁻CD45⁻CD133⁻ compared with CD34⁻Lin⁻CD45⁻CD133⁺ cells

Experiment no.	Purified starting cell population	Cells per well	No. of seeded wells	No. of wells with proliferated cells	No. of wells positive for cells
Experiment no.	Purified starting cell population	Cells per well	No. of seeded wells	No. of wells with proliferated cells	CD45⁺KDR⁻ only	CD45⁻KDR⁺ only	CD45⁺KDR⁻ CD45⁻KDR⁺
1	CD34⁻Lin⁻CD45⁻CD133⁻	1	60	12	8	1	3
	CD34⁻Lin⁻CD45⁻CD133⁻	10	6	6	3	1	2
1	CD34⁻Lin⁻CD45⁻CD133⁺	1	60	7	7	0	0
	CD34⁻Lin⁻CD45⁻CD133⁺	10	6	6	6	0	0
1	CD34⁻Lin⁻CD45⁺CD133⁻	1	60	14	14	0	0
	CD34⁻Lin⁻CD45⁺CD133⁻	10	6	6	6	0	0
1	CD34⁻Lin⁻CD45⁺CD133⁺	1	60	30	30	0	0
	CD34⁻Lin⁻CD45⁺CD133⁺	10	6	6	6	0	0

Experiment no.	Purified starting cell population	Cells per well	No. of seeded wells	No. of wells with proliferated cells	No. of wells positive for cells
Experiment no.	Purified starting cell population	Cells per well	No. of seeded wells	No. of wells with proliferated cells	CD45⁺KDR⁻ only	CD45⁻KDR⁺ only	CD45⁺KDR⁻ CD45⁻KDR⁺
1	CD34⁻Lin⁻CD45⁻CD133⁻	1	60	12	8	1	3
	CD34⁻Lin⁻CD45⁻CD133⁻	10	6	6	3	1	2
1	CD34⁻Lin⁻CD45⁻CD133⁺	1	60	7	7	0	0
	CD34⁻Lin⁻CD45⁻CD133⁺	10	6	6	6	0	0
1	CD34⁻Lin⁻CD45⁺CD133⁻	1	60	14	14	0	0
	CD34⁻Lin⁻CD45⁺CD133⁻	10	6	6	6	0	0
1	CD34⁻Lin⁻CD45⁺CD133⁺	1	60	30	30	0	0
	CD34⁻Lin⁻CD45⁺CD133⁺	10	6	6	6	0	0

Wells from 96-well plates precoated with MS-5 cells were seeded with 1-50 hPB cells of the selected phenotype with the use of the automatic cell device unit of the FACSVantage. Cells were cultured in LTC-IC-HE conditions as described in “Methods.” At the end of the 6-week culture period, individual wells were visually inspected, and wells that contained enough cells were harvested and cells were labeled with lineage-specific Abs. Analysis was performed on the FACSort as described.

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