Gene expression in freshly isolated B-lineage cell subsets from pediatric BM* and sorted CD19+CD127+ and CD19+CD127− cells from 4-week xenogeneic cultures
| Gene . | Probe set . | CD34+Lin− . | Pre-pro-B . | Pro-B . | Pre-B large . | Pre-B small . | Immature B . | CD127+ . | CD127− . | CD127− vs CD127+, -fold difference† . | CD127− vs CD127+, -fold difference† . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Survival/proliferation | |||||||||||
| CD127 (IL7R) | 226218_at | 114.5 | 681.6 | 377.1 | 919.8 | 706.5 | 630.8 | 5242.1 | 2074.0 | −2.5‡ | −5.6# |
| STAT5A | 203010_at | 1085.3 | 1201.7 | 693.3 | 456.5 | 523.2 | 375.9 | 468.4 | 448.5 | 1.0 | ND |
| STAT5B | 205026_at | 76.2 | 116.1 | 158.1 | 128.5 | 99.5 | 109.6 | 97.2 | 82.4 | −1.2 | ND |
| CCND2 | 200951_s_at | 332.5 | 377.9 | 167.5 | 130.9 | 99.0 | 117.1 | 334.5 | 135.9 | −2.5‡ | −5.0¶ |
| SLC2A1 | 201250_s_at | 263.0 | 255.5 | 295.3 | 409.3 | 370.1 | 458.3 | 376.9 | 244.8 | −1.5 | −1.4§ |
| BCL2 | 203685_at | 117.8 | 283.9 | 144.5 | 143.7 | 76.4 | 110.3 | 796.7 | 283.7 | −2.8‡ | ND |
| BCL6 | 203140_at | 383.8 | 1006.1 | 367.7 | 759.3 | 798.0 | 1292.0 | 470.6 | 645.0 | 1.4‡ | ND |
| MCL1 | 200797_s_at | 4372.5 | 5049.2 | 3264.3 | 4421.2 | 5347.8 | 4184.0 | 1786.4 | 1538.9 | −1.2 | ND |
| MKI67 | 212021_s_at | 134.1 | 533.0 | 1082.9 | 1562.2 | 578.9 | 1025.0 | 900.0 | 460.9 | −2.0‡ | ND |
| Ig rearrangement | |||||||||||
| IGH | 209374_s_at | 2814.2 | 5512.7 | 7720.9 | 13 182.0 | 14 308.4 | 14 294.9 | 4709.9 | 8866.8 | 1.9 | ND |
| IGKC | 224795_x_at | 437.2 | 320.3 | 4931.0 | 12 559.6 | 12 982.2 | 11 829.2 | 1698.8 | 5090.4 | 3.0‡ | 1.8§ |
| IGLC | 234764_x_at | 106.3 | 63.6 | 93.0 | 150.9 | 383.6 | 269.6 | 86.1 | 208.2 | 2.4‡ | ND |
| RAG1 | 206591_at | 38.1 | 99.7 | 780.6 | 146.0 | 534.5 | 51.8 | 898.9 | 3160.0 | 3.5‡ | 6.7# |
| RAG2 | 215117_at | 24.6 | 70.9 | 389.9 | 219.2 | 289.8 | 58.7 | 657.1 | 1186.5 | 1.8‡ | 1.9§ |
| LIG4 | 227766_at | 52.4 | 37.6 | 253.7 | 93.3 | 215.0 | 120.5 | 269.2 | 780.6 | 2.9‡ | ND |
| XRCC4 | 210813_s_at | 40.6 | 45.9 | 66.5 | 75.3 | 53.3 | 61.6 | 199.2 | 133.6 | −1.5‡ | ND |
| EZH2 | 203358_s_at | 216.6 | 1094.0 | 1627.9 | 1503.6 | 1046.8 | 1175.2 | 2763.2 | 2056.0 | −1.3‡ | −2.5 |
| DNTT | 210487_at | 117.9 | 9204.1 | 14 100.0 | 1860.8 | 2970.3 | 943.7 | 2728.3 | 4067.0 | 1.5 | 2.2§ |
| IGLL | 206660_at | 413.9 | 2378.5 | 6067.5 | 5344.0 | 3150.2 | 2447.8 | 2330.3 | 2730.3 | 1.2 | 1 |
| VPREB1 | 221349_at | 60.6 | 1365.2 | 4780.5 | 3257.6 | 3175.3 | 1882.2 | 1793.3 | 3753.6 | 2.1 | 1.8§ |
| Transcription factors | |||||||||||
| TCF3 | 209153_s_at | 648.9 | 1599.9 | 3078.6 | 3917.8 | 3805.8 | 1805.9 | 2259.9 | 4272.3 | 1.9‡ | 1.5§ |
| EBF1 | 229487_at | 134.2 | 980.4 | 1124.6 | 1072.3 | 946.3 | 744.0 | 1389.4 | 2245.7 | 1.6‡ | 1.3 |
| FOXO1 | 202723_s_at | 2784.6 | 1108.4 | 4723.5 | 3275.3 | 4808.2 | 3672.9 | 985.0 | 2758.4 | 2.8‡ | 2.7¶ |
| FOXO3 | 204131_s_at | 4404.1 | 4002.6 | 2646.4 | 691.8 | 1026.1 | 686.4 | 541.2 | 643.4 | 1.2 | 1.1 |
| ID2 | 201565_s_at | 1738.6 | 384.3 | 727.1 | 1071.5 | 761.5 | 864.9 | 1461.3 | 787.4 | −1.9‡ | −2.9§ |
| IKZF1 | 227346_at | 676.3 | 760.0 | 988.7 | 1139.8 | 885.1 | 1152.6 | 2956.7 | 3374.2 | 1.1 | −1.1 |
| IRF4 | 204562_at | 120.8 | 280.4 | 1068.1 | 4161.9 | 5581.8 | 3580.5 | 1179.8 | 2652.8 | 2.2‡ | 4.3§ |
| IRF8 | 204057_at | 530.5 | 911.0 | 560.4 | 729.6 | 543.7 | 2693.6 | 795.4 | 559.2 | −1.4 | −1.2 |
| PAX5 | 221969_at | 83.6 | 2709.9 | 4850.0 | 6006.2 | 7351.0 | 6083.7 | 4152.5 | 5177.0 | 1.2 | 1.2 |
| Other | |||||||||||
| CD19 | 206398_s_at | 100.1 | 310.3 | 2104.5 | 5010.5 | 6853.9 | 4997.7 | 3469.2 | 3621.7 | 1.0 | −1.5 |
| CD22 | 38521_at | 255.3 | 2038.1 | 1316.3 | 2086.9 | 1833.0 | 1841.4 | 732.3 | 958.5 | 1.3 | ND |
| CD79A | 205049_s_at | 190.4 | 3114.3 | 2194.5 | 5099.8 | 2450.2 | 3802.6 | 1748.8 | 1445.9 | −1.2 | 1 |
| CD79B | 205297_s_at | 154.3 | 2338.0 | 6444.1 | 8468.3 | 9375.5 | 4908.8 | 3257.4 | 7916.3 | 2.4‡ | ND |
| CD98 (SLC3A2) | 200924_s_at | 413.0 | 636.1 | 555.7 | 696.8 | 414.0 | 774.2 | 494.5 | 275.9 | −1.8 | ND |
| CD98 (SLC7A5) | 201195_s_at | 1215.8 | 1733.8 | 876.9 | 1697.9 | 1375.3 | 2014.7 | 968.1 | 151.0 | −6.4‡ | ND |
| Gene . | Probe set . | CD34+Lin− . | Pre-pro-B . | Pro-B . | Pre-B large . | Pre-B small . | Immature B . | CD127+ . | CD127− . | CD127− vs CD127+, -fold difference† . | CD127− vs CD127+, -fold difference† . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Survival/proliferation | |||||||||||
| CD127 (IL7R) | 226218_at | 114.5 | 681.6 | 377.1 | 919.8 | 706.5 | 630.8 | 5242.1 | 2074.0 | −2.5‡ | −5.6# |
| STAT5A | 203010_at | 1085.3 | 1201.7 | 693.3 | 456.5 | 523.2 | 375.9 | 468.4 | 448.5 | 1.0 | ND |
| STAT5B | 205026_at | 76.2 | 116.1 | 158.1 | 128.5 | 99.5 | 109.6 | 97.2 | 82.4 | −1.2 | ND |
| CCND2 | 200951_s_at | 332.5 | 377.9 | 167.5 | 130.9 | 99.0 | 117.1 | 334.5 | 135.9 | −2.5‡ | −5.0¶ |
| SLC2A1 | 201250_s_at | 263.0 | 255.5 | 295.3 | 409.3 | 370.1 | 458.3 | 376.9 | 244.8 | −1.5 | −1.4§ |
| BCL2 | 203685_at | 117.8 | 283.9 | 144.5 | 143.7 | 76.4 | 110.3 | 796.7 | 283.7 | −2.8‡ | ND |
| BCL6 | 203140_at | 383.8 | 1006.1 | 367.7 | 759.3 | 798.0 | 1292.0 | 470.6 | 645.0 | 1.4‡ | ND |
| MCL1 | 200797_s_at | 4372.5 | 5049.2 | 3264.3 | 4421.2 | 5347.8 | 4184.0 | 1786.4 | 1538.9 | −1.2 | ND |
| MKI67 | 212021_s_at | 134.1 | 533.0 | 1082.9 | 1562.2 | 578.9 | 1025.0 | 900.0 | 460.9 | −2.0‡ | ND |
| Ig rearrangement | |||||||||||
| IGH | 209374_s_at | 2814.2 | 5512.7 | 7720.9 | 13 182.0 | 14 308.4 | 14 294.9 | 4709.9 | 8866.8 | 1.9 | ND |
| IGKC | 224795_x_at | 437.2 | 320.3 | 4931.0 | 12 559.6 | 12 982.2 | 11 829.2 | 1698.8 | 5090.4 | 3.0‡ | 1.8§ |
| IGLC | 234764_x_at | 106.3 | 63.6 | 93.0 | 150.9 | 383.6 | 269.6 | 86.1 | 208.2 | 2.4‡ | ND |
| RAG1 | 206591_at | 38.1 | 99.7 | 780.6 | 146.0 | 534.5 | 51.8 | 898.9 | 3160.0 | 3.5‡ | 6.7# |
| RAG2 | 215117_at | 24.6 | 70.9 | 389.9 | 219.2 | 289.8 | 58.7 | 657.1 | 1186.5 | 1.8‡ | 1.9§ |
| LIG4 | 227766_at | 52.4 | 37.6 | 253.7 | 93.3 | 215.0 | 120.5 | 269.2 | 780.6 | 2.9‡ | ND |
| XRCC4 | 210813_s_at | 40.6 | 45.9 | 66.5 | 75.3 | 53.3 | 61.6 | 199.2 | 133.6 | −1.5‡ | ND |
| EZH2 | 203358_s_at | 216.6 | 1094.0 | 1627.9 | 1503.6 | 1046.8 | 1175.2 | 2763.2 | 2056.0 | −1.3‡ | −2.5 |
| DNTT | 210487_at | 117.9 | 9204.1 | 14 100.0 | 1860.8 | 2970.3 | 943.7 | 2728.3 | 4067.0 | 1.5 | 2.2§ |
| IGLL | 206660_at | 413.9 | 2378.5 | 6067.5 | 5344.0 | 3150.2 | 2447.8 | 2330.3 | 2730.3 | 1.2 | 1 |
| VPREB1 | 221349_at | 60.6 | 1365.2 | 4780.5 | 3257.6 | 3175.3 | 1882.2 | 1793.3 | 3753.6 | 2.1 | 1.8§ |
| Transcription factors | |||||||||||
| TCF3 | 209153_s_at | 648.9 | 1599.9 | 3078.6 | 3917.8 | 3805.8 | 1805.9 | 2259.9 | 4272.3 | 1.9‡ | 1.5§ |
| EBF1 | 229487_at | 134.2 | 980.4 | 1124.6 | 1072.3 | 946.3 | 744.0 | 1389.4 | 2245.7 | 1.6‡ | 1.3 |
| FOXO1 | 202723_s_at | 2784.6 | 1108.4 | 4723.5 | 3275.3 | 4808.2 | 3672.9 | 985.0 | 2758.4 | 2.8‡ | 2.7¶ |
| FOXO3 | 204131_s_at | 4404.1 | 4002.6 | 2646.4 | 691.8 | 1026.1 | 686.4 | 541.2 | 643.4 | 1.2 | 1.1 |
| ID2 | 201565_s_at | 1738.6 | 384.3 | 727.1 | 1071.5 | 761.5 | 864.9 | 1461.3 | 787.4 | −1.9‡ | −2.9§ |
| IKZF1 | 227346_at | 676.3 | 760.0 | 988.7 | 1139.8 | 885.1 | 1152.6 | 2956.7 | 3374.2 | 1.1 | −1.1 |
| IRF4 | 204562_at | 120.8 | 280.4 | 1068.1 | 4161.9 | 5581.8 | 3580.5 | 1179.8 | 2652.8 | 2.2‡ | 4.3§ |
| IRF8 | 204057_at | 530.5 | 911.0 | 560.4 | 729.6 | 543.7 | 2693.6 | 795.4 | 559.2 | −1.4 | −1.2 |
| PAX5 | 221969_at | 83.6 | 2709.9 | 4850.0 | 6006.2 | 7351.0 | 6083.7 | 4152.5 | 5177.0 | 1.2 | 1.2 |
| Other | |||||||||||
| CD19 | 206398_s_at | 100.1 | 310.3 | 2104.5 | 5010.5 | 6853.9 | 4997.7 | 3469.2 | 3621.7 | 1.0 | −1.5 |
| CD22 | 38521_at | 255.3 | 2038.1 | 1316.3 | 2086.9 | 1833.0 | 1841.4 | 732.3 | 958.5 | 1.3 | ND |
| CD79A | 205049_s_at | 190.4 | 3114.3 | 2194.5 | 5099.8 | 2450.2 | 3802.6 | 1748.8 | 1445.9 | −1.2 | 1 |
| CD79B | 205297_s_at | 154.3 | 2338.0 | 6444.1 | 8468.3 | 9375.5 | 4908.8 | 3257.4 | 7916.3 | 2.4‡ | ND |
| CD98 (SLC3A2) | 200924_s_at | 413.0 | 636.1 | 555.7 | 696.8 | 414.0 | 774.2 | 494.5 | 275.9 | −1.8 | ND |
| CD98 (SLC7A5) | 201195_s_at | 1215.8 | 1733.8 | 876.9 | 1697.9 | 1375.3 | 2014.7 | 968.1 | 151.0 | −6.4‡ | ND |
ND indicates not determined.
The results for CD34+Lin−, pre-pro-B, pro-B, pre-B large, pre-B small, and immature B were obtained from a new analysis on previously published data.25
The -fold difference was calculated by determining the absolute value of the ratio of transcript expression between each population. Values were assigned a negative value if gene expression was higher in CD127+ compared with CD127− and values were assigned a positive value if gene expression was higher in CD127− compared with CD127+. Left column is microarray data and right column is quantitative RT-PCR data.
Statistically significant difference between CD127+ and CD127− cells based on DNA microarray data.
P < .05 as determined by Student t test on quantitative RT-PCR data.
P < .01 as determined by Student t test on quantitative RT-PCR data.
P < .001 as determined by Student t test on quantitative RT-PCR data.