Cellular distribution of Btk, p-Btk, Lyn, and p-Lyn in BM sections in patients with systemic mastocytosis* as determined by IHC
| . | Reactivity of BM cells with Abs against . | |||
|---|---|---|---|---|
| Lyn . | p-LynTyr507 . | Btk . | p-BtkTyr223 . | |
| Megakaryocytes | + | + | − | − |
| Myeloid progenitors | ± | + | − | − |
| Neutrophil granulocytes | ± | + | − | − |
| Eosinophil granulocytes | − | − | − | − |
| Erythroid progenitors | ± | ± | − | − |
| Lymphocytes | ± | ± | ± | ± |
| MCs | + | + | + | + |
| . | Reactivity of BM cells with Abs against . | |||
|---|---|---|---|---|
| Lyn . | p-LynTyr507 . | Btk . | p-BtkTyr223 . | |
| Megakaryocytes | + | + | − | − |
| Myeloid progenitors | ± | + | − | − |
| Neutrophil granulocytes | ± | + | − | − |
| Eosinophil granulocytes | − | − | − | − |
| Erythroid progenitors | ± | ± | − | − |
| Lymphocytes | ± | ± | ± | ± |
| MCs | + | + | + | + |
BM sections were stained with Abs against signaling molecules as described in “IHC and ICC.” Score: − indicates negative; +, positive; and ±, subset of cells positive.
MC indicates mast cell.
The same results were obtained with sections of normal BM as well as BM sections obtained from patients with CML (exception: MCs were only examined in mastocytosis).