Relative expression of IFN-γ–inducible genes in erythroblasts of CD70TG mice and their relationship with IRF-1
| IRF-1–related gene . | Fold difference in expression between . | Relationship with IRF-1 . | ||||
|---|---|---|---|---|---|---|
| CD70TG vs WT . | CD70TG vs CD70TG*IFN-γ−/− . | WT vs IFN-γ−/− . | WT vs CD70TG*IFN-γ−/− . | IFN-γ−/− vs CD70TG*IFN-γ−/− . | ||
| IRF-1 | 3.0 | 5.0 | 1.1 | 1.6 | 1.5 | IRF-1 itself |
| CD274 (PD-L1) | 2.1 | 2.1 | −1.0 | 1.0 | 1.0 | IRF-1–dependent IFN-γ–inducible gene30 |
| GBP1 | 1.9 | 1.9 | 1.0 | 1.0 | 1.0 | IRF-1–dependent IFN-γ–inducible gene31 |
| GBP2 | 12.2 | 21.5 | 1.4 | 1.8 | 1.3 | IRF-1–dependent IFN-γ–inducible gene32 |
| GBP4 | 4.0 | 5.9 | 1.1 | 1.5 | 1.3 | IRF-1–dependent IFN-γ–inducible gene32 |
| PSMB9 (LMP-2) | 3.2 | 4.3 | 1.1 | 1.3 | 1.2 | IRF-1–dependent IFN-γ–inducible gene33 |
| TAP1 | 3.3 | 4.6 | 1.1 | 1.4 | 1.2 | IRF-1–dependent IFN-γ–inducible gene33 |
| IFI47 (IRG47) | 3.0 | 3.6 | −1.0 | 1.2 | 1.2 | IRF-1–independent IFN-γ–inducible gene32 |
| IGTP | 4.1 | 6.5 | −1.1 | 1.6 | 1.7 | IRF-1–independent IFN-γ–inducible gene32 |
| IIGP1 | 12.7 | 14.7 | 1.2 | 1.2 | 1.0 | IRF-1–independent IFN-γ–inducible gene32 |
| IRGM (LRG47) | 1.7 | 2.3 | −1.1 | 1.3 | 1.5 | IRF-1–independent IFN-γ–inducible gene32 |
| TGTP | 4.9 | 8.8 | 1.1 | 1.9 | 1.7 | IRF-1–independent IFN-γ–inducible gene32 |
| IRF-1–related gene . | Fold difference in expression between . | Relationship with IRF-1 . | ||||
|---|---|---|---|---|---|---|
| CD70TG vs WT . | CD70TG vs CD70TG*IFN-γ−/− . | WT vs IFN-γ−/− . | WT vs CD70TG*IFN-γ−/− . | IFN-γ−/− vs CD70TG*IFN-γ−/− . | ||
| IRF-1 | 3.0 | 5.0 | 1.1 | 1.6 | 1.5 | IRF-1 itself |
| CD274 (PD-L1) | 2.1 | 2.1 | −1.0 | 1.0 | 1.0 | IRF-1–dependent IFN-γ–inducible gene30 |
| GBP1 | 1.9 | 1.9 | 1.0 | 1.0 | 1.0 | IRF-1–dependent IFN-γ–inducible gene31 |
| GBP2 | 12.2 | 21.5 | 1.4 | 1.8 | 1.3 | IRF-1–dependent IFN-γ–inducible gene32 |
| GBP4 | 4.0 | 5.9 | 1.1 | 1.5 | 1.3 | IRF-1–dependent IFN-γ–inducible gene32 |
| PSMB9 (LMP-2) | 3.2 | 4.3 | 1.1 | 1.3 | 1.2 | IRF-1–dependent IFN-γ–inducible gene33 |
| TAP1 | 3.3 | 4.6 | 1.1 | 1.4 | 1.2 | IRF-1–dependent IFN-γ–inducible gene33 |
| IFI47 (IRG47) | 3.0 | 3.6 | −1.0 | 1.2 | 1.2 | IRF-1–independent IFN-γ–inducible gene32 |
| IGTP | 4.1 | 6.5 | −1.1 | 1.6 | 1.7 | IRF-1–independent IFN-γ–inducible gene32 |
| IIGP1 | 12.7 | 14.7 | 1.2 | 1.2 | 1.0 | IRF-1–independent IFN-γ–inducible gene32 |
| IRGM (LRG47) | 1.7 | 2.3 | −1.1 | 1.3 | 1.5 | IRF-1–independent IFN-γ–inducible gene32 |
| TGTP | 4.9 | 8.8 | 1.1 | 1.9 | 1.7 | IRF-1–independent IFN-γ–inducible gene32 |
Selection of IRF-1–related genes (based on the microarray data described in Figure 4 and supplemental Figure 3) that were significantly up-regulated in CD70TG mice compared with WT as well as CD70TG*IFN-γ−/− mice. These IFN-γ–inducible genes were categorized as being either IRF-1–dependent (top half) or –independent (bottom half), based on indicated literature references (right column).